早期干预对宫内缺氧、缺血大鼠脑功能及神经生长相关蛋白表达的影响

目的　研究早期干预对宫内缺氧、缺血大鼠脑功能及神经生长相关蛋白表达的影响。方法　选用SD大鼠制作宫内缺氧、缺血脑损伤 (hypoxic ischemicbraindamage ,HIBD)动物模型共计 3 0只 ,并同时选取 3 0只正常大鼠作为对照。将上述大鼠随机分为HIBD干预组、HIBD非干预组、正常干预组及正常非干预组。各干预组大鼠于出生后 2～ 2 8d期间 ,分别对其进行早期干预 (包括按摩、丰富环境刺激等 )。当早期干预治疗结束后 ,通过跳台测试评价各组大鼠的脑功能 ,随后取各组大鼠脑组织进行GAP 43免疫组化染色检测 ,以观察各组大鼠GAP 43表达的差异。结果　在跳台测试实验中 ,HIBD干预组大鼠学习、记忆成绩明显优于HIBD非干预组 (P <0 .0 5 ) ,正常干预组大鼠的学习、记忆成绩也明显优于正常非干预组 (P <0 .0 5 ) ;通过GAP 43免疫组化检测结果发现 ,HIBD干预组大鼠额皮质及海马CA3区的GAP 43表达明显强于HIBD非干预组 (P <0 .0 5 ) ,正常干预组的GAP 43表达亦明显强于正常非干预组 (P <0 .0 5 )。结论　早期干预可以促进大鼠受损脑功能的恢复 ,而且对正常大鼠的脑功能发育也有促进作用 ,GAP 43表达的增强可能是早期干预改善大鼠脑功能的主要机制之一。

Effects of early intervention on functional outcome and content of GAP-43 in the brain of rats with hypoxic-ischemic brain damage

Objective To study the effects of early intervention on functional outcome and content of growth associated protein (GAP- 43) in the brain of rats with hypoxic-ischemic brain damage (HIBD). Methods The animal model of intra-uterus HIBD was established in 30 pregnant Sprague-Dawley rats, who were then divided randomly into 4 groups: the intervention HIBD group (HIBD-IT),the non-intervention HIBD group (HIBD-NIT),the intervention control group(control-IT),the non-intervention control group(control-NIT). From the second day after the operation, the rats of the HIBD-IT and control-IT groups were kept in an enriched environment and administered with the neonatal handling including massage for 14 days. On the 28th day, jumping stand test was administered to all the rats, at the same time, the expression of GAP- 43 in brain of the rats in different groups were measured by immunohistochemical method. Results On the jumping stand test, the performance of control-IT and HIBD-IT groups were significantly better than that of the control-NIT and HIBD-NIT groups(P<0.05), respectively. Immunohistochemical analysis showed that expression of GAP- 43 in prefrontal cortex and CA3 region of hippocampus, was significantly stronger in the HIBD-IT and control-IT groups as compared with that of the HIBD-NIT and the control-NIT groups (P<0.05), respectively. Conclusion Early intervention could effectively improve learning and memory and relieve the neuron behavior sequela in HIBD rats, and the intervention could also improve the brain function of the normal rats. The up-regulated GAP- 43 expression in the brain may contributed to the amelioration of brain function treated by early intervention.