vasohibin基因重组腺病毒对胶质瘤U251移植瘤的抑制作用

目的探讨vasohibin基因重组腺病毒对胶质瘤U251移植瘤的抑制作用。方法构建vasohibin基因重组腺病毒,观察其对人脐静脉血管内皮细胞(HUVEC)体外增殖的影响。建立胶质瘤U251移植瘤模型,观察vasohibin基因重组腺病毒对胶质瘤U251移植瘤生长的抑制作用。结果 vasohibin基因重组腺病毒能明显抑制HUVEC的增殖,显著减缓胶质瘤U251移植瘤生长(P<0.05),同时明显减少了移植瘤组织微血管密度(P<0.05)。结论 vasohibin基因重组腺病毒能明显抑制胶质瘤U251移植瘤的生长与血管生成,为胶质瘤的基因治疗提供了新的思路。

Inhibitory effects of recombinant adenovirus carrying vasohibin on the growth of glioma U251 cells

Objective To evaluate the inhibitory effect of recombinant adenovirus carrying vasohibin（Ad-vasohibin）on theg rowth of glioma U251 cells.Methods The effect of Ad-vasohibin,which was prepared,on the proliferation of human umbilical veine ndothelial cells（HUVECs）in vitro was determined by methyl thiazolyl tetrazolium（MTT）technique.The inhibitory effect ofA d-vasohibin on the gliomas,which were established by U251 cells xenograft in the nude mice,were observed.The intratumoralm icrovessel density（MVD）was evaluated by CD31 staining.Results HUVECs proliferation and the growth of U251 cell xenograftg liomas were significantly inhibited by in Ad-vasohibin compared to the corresponding control groups（P0.05）.The MVD decreaseds ignificantly in Ad-vasohibin treatment group compared to that in the control group（P0.05）.Conclusion That Ad-vasohibin cani nhibit the growth of glioma U251 cells xenograft may provide a new method for the gene treatment of the glioma.