三叉神经根区粘连蛛网膜组织中IL-6的表达及意义

目的探讨原发性三叉神经痛(TN)患者三叉神经根区粘连的蛛网膜组织中白细胞介素-6(IL-6)表达对单纯疱疹病毒Ⅰ型(HSV-1)的影响,并进一步探讨HSV-1感染与TN的关系。方法首先采用PCR方法检测TN患者三叉神经根区粘连的蛛网膜、无粘连的蛛网膜组织中HSV-1特异性基因片段,再用免疫印迹法分别检测上述组织中抗原的表达,从而确定病毒感染状态,将其重新划分为HSV-1潜伏感染组、增殖感染组、未感染组,再用酶联免疫吸附测定法分别定量检测三组蛛网膜标本组织和患者血清中IL-6水平。结果 HSV-1增殖感染组的蛛网膜组织中IL-6水平(324.64±14.28)pg/g]高于潜伏感染组(232.75±19.17)pg/g],潜伏感染组也高于未感染组(93.54±14.08)pg/g],且均有统计学差异(P<0.01);血清中IL-6水平三组之间无明显差异(P>0.05)。结论 IL-6是HSV-1增殖感染过程中的重要介质。HSV-1的增殖感染可能诱发或加重三叉神经根区蛛网膜的粘连,蛛网膜组织可能也是HSV-1的潜伏基地。HSV-1感染可能是继血管压迫之外造成三叉神经根区蛛网膜粘连继而引发TN的另一重要因素。

Expression of IL-6 in arachnoidal tissues adhering to the trigeminal nerve entry root zone and its significance in patients with trigeminal neuralgia

Objectives To investigate the effect of Interleukin-6 expression in the arachnoidal tissues adhering to the trigeminaln erve entry root zones（ERZ）on herpes simplex virus type 1（HSV-1）in the patients with trigeminal neuralgia（TN）and to understand ther elationship between HSV-1 infection and TN.Methods The HSV-1 specific DNA fragments in the arachnoidal tissues including thet issues adhering or not adhering to the trigeminal nerve ERZ were detected by polymerase chain reaction（PCR）in 79 patients with TN.T he HSV-1 specific antigen was detected by Western Blotting technique in order to determine the infection status,according to which7 9 patients were divided into the latent infection group,reactive infection group and uninfected group.And then the serum anda rachnoidal tissues levels of IL-6 expression in the all the patients were quantitatively determined by enzyme-labeled immunosorbenta ssay.Results The arachnoidal tissues level of IL-6（324.64±14.28）pg/g]in the HSV-1 reactive infection group was significantly highert han that（232.75±19.17）pg/g]in the latent infection group（P0.01）,which was significantly higher than that（93.54±14.08）pg/g]in theu ninfected group（P0.01）.There was insignificant difference in the serum levels of IL-6 among the reactive infection,latent infectiona nd uninfected groups（P0.05）.Conclusions IL-6 is an important cytokine in the process of HSV-1 reactive infection.The reactivei nfection of HSV-1 may induce or aggravate arachnoidal tissues adhesion to the trigeminal nerve ERZ.The arachnoidal tissues may bea nother latent HSV-1 base.It is suggested besides the vascular compression,HSV-1 infection may be another important factor related toT N.