| 硝酸异山梨酯对大鼠膀胱逼尿肌的舒张作用 |
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| 引用本文： | 王占庆,王金萍,许景峰,曾明,陈艳.硝酸异山梨酯对大鼠膀胱逼尿肌的舒张作用[J].中国临床药理学与治疗学,2011,16(11):1248-1251. |
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| 作者姓名： | 王占庆 王金萍 许景峰 曾明 陈艳 |
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| 作者单位： | 北京军区总医院药理科,北京,100700 |
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| 摘 要： | 目的:研究硝酸异山梨酯(ISDN)对大鼠离体膀胱逼尿肌螺旋条的舒张作用及可能机制。方法:采用离体逼尿肌螺旋条浴槽实验方法,以硝苯地平为对照,测定ISDN(10-6～10-4 mol/L)对氯化钾(4×10-2 mol/L)和乙酰胆碱(10-4 mol/L)预收缩离体膀胱逼尿肌条的平均张力、收缩幅度和频度的影响。用标准试剂盒测定膀胱组织经ISDN(10-4 mol/L)孵育1h后组织中一氧化氮(NO)和一氧化氮合酶(NOS)的活性。结果:ISDN的中剂量组和高剂量组(10-5 mol/L和10-4 mol/L)对乙酰胆碱预收缩肌螺旋条的平均张力、收缩幅度和频度均明显降低(P<0.05);ISDN各剂量组对高钾预收缩膀胱逼尿肌条的平均张力、收缩幅度和频度均没有明显影响(P>0.05)。另外,膀胱组织经ISDN孵育后NO的含量和NOS的活性明显升高(P<0.05)。结论:ISDN对乙酰胆碱预收缩的膀胱逼尿肌条有一定舒张作用,其机制可能与ISDN激活NOS,升高NO的含量有关。
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| 关 键 词： | 硝酸异山梨酯 膀胱逼尿肌 舒张作用 一氧化氮合酶 大鼠 |
Effects of isosorbide dinitrate on contractions of rat urine bladder destrusor in vitro |
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| WANG Zhan-qing,WANG Jin-ping,XU Jing-feng,ZENG Ming,CHEN Yan.Effects of isosorbide dinitrate on contractions of rat urine bladder destrusor in vitro[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2011,16(11):1248-1251. |
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| Authors: | WANG Zhan-qing WANG Jin-ping XU Jing-feng ZENG Ming CHEN Yan |
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| Institution: | WANG Zhan-qing,WANG Jin-ping,XU Jing-feng,ZENG Ming,CHEN Yan Department of Pharmacology,General Hospital of Beijing Military Command,Beijing 100700,China |
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| Abstract: | AIM:To investigate the dilation effect of isosorbide dinitrate(ISDN) on the rat urine bladder rings.METHODS: The values of the mean contraction tension,maximum amplitude and frequency of rat urine bladder rings were measured.The activity of nitric oxide synthase(NOS) and the content of nitric oxide(NO) were measured in the rat urine bladder tissue pre-incubated with ISDN(10-4 mol/L) for 1 h.RESULTS:ISDN(10-5 mol/L,10-4 mol/L) could inhibit the mean contractile tension,maximum amplitude and frequency of spon... |
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| Keywords: | Isosorbide dinitrate Urine bladder detrusor Dilation Nitric oxide synthase Rat |
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