HPV16 E6/E7对永生化口腔上皮细胞p53、p21表达的影响

目的：为了探讨人乳头状瘤病毒(HPV)转化口腔上皮细胞的作用机制，研究HPV16 E6、E7对永生化口腔上皮细胞细胞周期调节因子p53、p21表达的影响。方法：免疫细胞化学法检测HPV16 E6、E7诱导的永生化口腔上皮细胞HIOEC中野生型和突变型p53的表达；Westem印迹检测HIOEC HPV16 E6、E7、p53、p21蛋白表达，并以正常口腔上皮细胞和转染空白载体的口腔上皮细胞为对照。结果：HIOEC细胞表达HPV16 E6、E7蛋白．野生型p53和p21表达水平高于正常口腔上皮细胞和转染空白载体的口腔上皮细胞。结论：p53的失活可能不是HPV16 E6、E7诱导口腔上皮细胞永生化的主要原因。

Influence of HPV16 E6 and E7 on expression of p53 and p21 in oral epithelial cell

PURPOSE:To investigate the role of HPV16E6and E7during the transformation of oral epithelial cells.METHODS:An human immortalized oral epithelial cell line HIOEC was established by transfecting HPV16E6/E7open reading frames using recombinant retroviral system pLXSN to human normal oral epithelial cells.Expression of wild-type and mutant p53were analyzed by immunocytochemical staining in HIOEC and human normal oral epithelial cells.Expression of HPV16E6,E7,p53and p21were analyzed in HIOEC,human normal oral epithelial cells and the oral epithelial cells transfected with pLXSN were used as control groups.RESULTS:HIOEC expressed HPV16E6and E7.Compared with the two control groups,E6and E7expression were correlated with increased levels of the p53,p21in HIO615.CONCLUSION:The inactivation of p53was not the reason of immortalization of human oral epithelial cells induced by HPV16E6and E7.