HIF-1α与TRAIL-DR4在宫颈癌组织中的表达及与放疗敏感性的研究

目的:研究缺氧诱导因子-1α(HIF-1α)及肿瘤坏死因子相关的凋亡诱导配体(TRAIL)受体在宫颈癌组织中的表达变化,及与放疗敏感性的关系。方法:收集2003年7月至2005年1月宫颈癌活检组织45例,用原位杂交组织化学法检测宫颈癌组织中HIF-1αmRNA及TRAIL-DR4mRNA水平。结果:(1)在45例宫颈癌组织中27例(60%) HIF-1αmRNA呈阳性表达,Ⅰ/Ⅱ期肿瘤HIF-1αmRNA的表达显著低于Ⅲ/Ⅳ期肿瘤的表达(X~2=9．07,P＜0．05),随着组织分化的降低,HIF-1αmRNA的表达增强(X~2=8．03,P＜0．05),放疗敏感患者中HIF-1αmRNA表达明显低于放疗不敏感患者(X~2=10．37,P＜0．05);(2)TRAIL-DR4mRNA阳性表达率为57．8%。Ⅰ/Ⅱ期肿瘤DR4mRNA的表达显著高于Ⅲ/Ⅳ期肿瘤的表达(X~2=9．01,P＜0．05),中高分化宫颈癌的表达明显高于低分化宫颈癌(X~2=18．18,P＜0．05),放疗敏感患者中TRAIL-DR4mRNA表达明显高于放疗不敏感患者(X~2=6．91,PP＜0．05)。结论:HIF-1αmRNA及TRAIL-DR4mRNA与宫颈癌的放疗敏感有相关性。

Expressions of HIF-1α and TRAIL-DR4 in cervical cancer and their relationships with radiosensitivity

Objective:To study the expressions of hypoxia-inducible factor-1αand TNF related apoptosis inducing ligand receptor in cervical cancer tissue and their relationships with radiosensitivity.Method:Forty-five samples from patients with cervical cancer were as- sessed for HIF-1αand TRAIL-DR4 mRNA expression by in situ hybridization histochemistry (ISHH).Rusult:(1)HIF-1αmRNA was over expressed in 27 samples(60%).The level of expression in early stages(ⅠandⅡ)cases was lower than that in advanced stages(ⅢandⅣ)cases(X~2=9.07,P＜0.05).The expression of HIF-1αelevated as the pathologic grade de- creased(X~2=8.03,P＜0.05).Patients who had complete response to radiotherapy were found to have lower HIF-1αmRNA expression than those who were not sensitive to radiotherapy(X~2= 10.37,P＜0.05).(2)The positive rate of DR4mRNA was 57.8%.The level of expression in early stages(ⅠandⅡ)cases was higher than that in advanced stages(ⅢandⅣ)cases(X~2= 9.01,P＜0.05).The positive rate of DR4mRNA expression in well-differentiated cases was significandy higher than that in poorly differentiated cases.(X~2=18.18,P＜0.05).Patients who showed complete response to radiotherapy were found to have higher DR4mRNA expres- sion,than those who showed incomplete response to radiotherapy(X~2=6.91,P＜0.05).Con- clusion:The expression of HIF-1αand TRAIL-DR4 are related to radiosensitivity.