| Aβ25-35对PC12细胞凋亡蛋白Bcl-2和Bax表达的影响 |
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| 引用本文： | 张美,狄婷婷,王瑞婷.Aβ25-35对PC12细胞凋亡蛋白Bcl-2和Bax表达的影响[J].承德医学院学报,2016,33(2). |
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| 作者姓名： | 张美 狄婷婷 王瑞婷 |
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| 作者单位： | 承德医学院,河北承德,067000;承德护理职业学院 |
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| 基金项目： | 河北省教育厅重点资助课题(ZD20131051)，河北省中医药抗痴呆重点研究室建设项目(20142803)，河北省高校重点学科资助项目 |
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| 摘 要： | 目的：：观察Aβ25-35对PC12细胞凋亡蛋白Bcl-2和Bax表达的影响。方法：采用10μmol/L Aβ25-35与PC12细胞共孵育48h建立阿尔茨海默病细胞模型，对照组细胞不加Aβ25-35。采用Hoechst 33258核染色法检测PC12细胞的凋亡情况，免疫细胞化学染色法检测PC12细胞Bcl-2和Bax蛋白的表达情况。结果：与对照组相比，实验组细胞Bcl-2的表达明显降低，细胞凋亡率、Bax蛋白的表达和Bax/Bcl-2比值明显升高(P＜0.05）。结论：Aβ25-35诱导PC12细胞凋亡的机制可能与下调Bcl-2表达、上调Bax表达有关。
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| 关 键 词： | β淀粉样蛋白 PC12细胞 凋亡 Bcl-2 Bax |
EFFECTS OF AMYLOID BETA-PROTEIN(25-35) ON APOPTOTIC PROTEIN BCL-2 AND BAX EXPRESSION IN PC12 CELLS |
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| ZHANG Mei,DI Ting-ting,WANG Rui-ting.EFFECTS OF AMYLOID BETA-PROTEIN(25-35) ON APOPTOTIC PROTEIN BCL-2 AND BAX EXPRESSION IN PC12 CELLS[J].Journal of Chengde Medical College,2016,33(2). |
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| Authors: | ZHANG Mei DI Ting-ting WANG Rui-ting |
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| Abstract: | ABSTRACT]Objective:To explore the effects of amyloid beta-protein (25-35) on Bcl-2 and Bax expression in PC12 cell. Methods:Alzheimer’s disease cells model was made by culturing the PC12 cells with 10μmol/L Aβ25-35 for 48 hours. Hoechst 33258 method was used to detect the apotosis of PC12 cells, and immunocytochemical stain was used to detect the expression of Bcl-2 and Bax protein in PC cells. Results:Compared with control group, the Bcl-2 expression in PC12 cells of experimental group decreased obviously, while the apoptosis rate, Bax expression and Bax/Bcl-2 increased obviously (P<0.05). Conclusions:The mechanisms of PC12 cells apoptosis induced by Aβ25-35 may be related to down-regulation of Bcl-2 expression and up-regulation of Bax expression. |
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| Keywords: | Amyloid beta-protein PC12 cell Apoptosis Bcl-2 Bax |
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