SCI大鼠脊髓前角细胞自噬相关蛋白Beclin-1表达的变化

目的：：观察脊髓全横断损伤对脊髓前角细胞Beclin-1表达的影响。方法：雄性Wistar大鼠随机分为模型组和假手术组，建立大鼠第2腰髓全横断损伤模型，分别于损伤后6h、1d、3d、7d、14d、21d取3-4腰髓，采用免疫荧光染色法观察大鼠脊髓前角细胞自噬相关蛋白Beclin-1的表达情况。结果：与假手术组相比，模型组损伤后各时间点大鼠脊髓前角Beclin-1免疫阳性细胞数明显升高(P＜0.01，P＜0.05）。脊髓损伤后3d脊髓前角细胞Beclin-1免疫阳性细胞数达到高峰，21d最低。结论：Beclin-1可能参与了神经元自噬状态的改变。

CHANGES OF AUTOPHAGY RELATED PROTEIN BECLIN-1 EXPRESSION IN ANTERIOR HORN CELLS OF SPINAL CORD INJURY RATS

[ABSTRACT]Objective:To observe the effects of complete transection of spinal cord on Beclin-1 expression in anterior horn cells of rats’ spinal cord. Methods:Male Wistar rats were randomly divided into model group and sham-operation group. The spinal cord injury (SCI) rat’s model were made by completely transecting the 2nd lumbar spinal cord. Immunolfuorescent staining was used to detect the Beclin-1 protein expression in anterior horn cells of the 3rd-4th lumbar spinal cord 6h, 1d, 3d, 7d, 14d and 21d after injury respectively. Results:Compared with rats in sham-operation group, the Beclin-1 positive cells in anterior horn of spinal cord of rats in model group at each time point increased obviously (P<0.01, P<0.05). The Beclin-1 positive cells in anterior horn of spinal cord reached the peak 3 days after injury, and reached the lowest 21 days after injury. Conclusions:Beclin-1 may be involved in the changes of neuron autophagy.