同时使用三种细胞对急性弛缓性麻痹病例病原学监测结果的影响探讨

山西省卫生防疫站脊髓灰质炎(脊灰)实验室从1998年7月正式使用L20B细胞,并同时使用RD和Hep-2细胞进行急性弛缓性麻痹(AFP)病例病原学监测,为了解同时使用3种细胞对AFP病例病原学监测结果的影响,将308例AFP病例粪便标本在3种细胞上的分离状况进行比较分析.同时使用3种细胞共分离到脊灰病毒(PV)20份,其中RD细胞分离到14份,L20B细胞16份,Hep-2细胞8份,总分离率为6.5%.分离到非脊灰肠道病毒(NPEV)65株,其中RD细胞30株,Hep-2细胞48株,分离率为21.1%.结果显示,同时使用3种细胞对提高PV检测灵敏度和NPEV分离率,保证脊灰实验室监测质量有重要意义.

Simultaneous Use of 3 Cell Types for Virological Surveillance of Acute FlaccidParalysis Cases

The L20B cell was formally used in our polio laboratory since July 1998,RD and Hep-2 cells were simultaneously used for acute flaccid paralysis(AFP)cases surveillance 308 samples of AFP cases were inoculated onto 3 types of cells simultaneously The results of virus isolation were compared and analyzed 20 polioviruses in total were isolated,the positive rate was 6 5%,of which 14,16 and 8 were isolated in RD,L20B and Hep-2 cell respectively 65 non-polio enteroviruses(NPEV)were isolated from AFP cases,30 in RD and 48 in Hep-2,the positive rate was 21 1%. Using 3 kinds of cells simutaneously are very necessary for enhancing the sensitivity of AFP virological surveillance,the isolation rate of NPEV,and to ensure the surveillance quality of poliovirus laboratory