bcl-2蛋白在肝细胞肝癌发生过程中的表达及其与肝细胞凋亡变化的关系

为探讨肝细胞肝癌发生过程中ｂｃｌ２蛋白的变化规律及与细胞凋亡变化的关系，运用ＴＵＮＥＬ法和免疫组化方法检测石蜡保存的肝细胞肝癌组织（ｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａ，ＨＣＣ）、肝硬变组织（ｌｉｖｅｒｃｉｒｈｏｓｉｓ，ＬＣ）和正常肝组织（ｎｏｒｍａｌｌｉｖｅｒ，ＮＬ）中细胞凋亡发生率和ｂｃｌ２的表达。结果：从ＮＬ、ＬＣ到ＨＣＣ组织，细胞凋亡活性出现增强；ｂｃｌ２蛋白的表达逐渐下降，ＬＣ和ＨＣＣ组比较，Ｐ＜０．０５。结论：ｂｃｌ２的表达可能与肝细胞凋亡的抑制有关；在肝癌发生过程中，ｂｃｌ２表达下降可能是引起肝癌细胞凋亡增加的原因之一。

BCL-2 EXPRESSION IN THE HEPATOCELLULAR CARCINOGENETIC PROCESS AND ITS RELATIONSHIP WITH HEPATIC CELL APOPTOSIS

To elucidate bcl2 protein expression in hepatic carcinogenetic process and its relationship with apoptotic changes. bcl2 protein was evaluated immunohistochemically while apoptosis was approached with terminal deoxynucleotidyl transferase (TdT)mediated dUTP nick end labeling (TUNEL) technique in 8 normal livers (NL), 17 liver cirrhosises (LC) and 77 hepatocellular carcinomas (HCC). The results showed that bcl2 protein was expressed in 3 of 8 NLs(37.5%), 5 of 17 LCs(29.4%) and 7 of 77 HCCs(9.1%) with significant differences between group NL and HCC and between LC and HCC (P<0.05). Apoptosis rates of 1.180.42%, 4.852.78%, 12.892.33% in NL, LC, HCC group respectively were demonstrated with significant differences among them (P<0.01). Compared the bcl2 expression with the apoptosis rate in this hepatocarcinogenetic process, reversed trends were presented. Conclusion: bcl2 expression could be detected in NL, LC and HCC, and its decreasing expression was related to the inhibition attenuation of hepatocell