| 中药材厚朴的DNA扩增产物指纹分析研究 |
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| 引用本文： | 王艇,苏应娟,朱建明,李雪雁,曾庆文,夏念和.中药材厚朴的DNA扩增产物指纹分析研究[J].中药材,2001,24(10):710-715. |
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| 作者姓名： | 王艇 苏应娟 朱建明 李雪雁 曾庆文 夏念和 |
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| 作者单位： | 1. 中山大学生命科学院,
2. 中国科学院华南植物研究所, |
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| 摘 要： | 采用DNA扩增产物指纹分析（DNA amplification fingerprinting,DAF)技术鉴别中药材厚朴、凹叶厚朴及其常见的伪品、混淆品、获得了清晰可靠的DNA指纹图谱。根据聚丙烯酰胺凝胶上显示的DNA带型差异可简便地区分出厚朴、凹叶厚朴和其它伪品、混淆品。证明DNF技术可以准确、快速地鉴别出中药材厚朴及其伪、混品、在正确引种方面具有较高的价值。
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| 关 键 词： | 厚朴 DAF 伪品 混淆品 中药材 DNA扩增产物 指纹分析 |
| 修稿时间： | 2001年7月3日 |
Studies on DNA Amplification Fingerprinting of Cortex Magnoliae Officinalis |
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| Wang Ting,Su Yingjuan,Zhu Jianming,Li Xueyan,Zeng Qingwen,Xia Nianhe.Studies on DNA Amplification Fingerprinting of Cortex Magnoliae Officinalis[J].Jorunal of Chinese Medicinal Materials,2001,24(10):710-715. |
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| Authors: | Wang Ting Su Yingjuan Zhu Jianming Li Xueyan Zeng Qingwen Xia Nianhe |
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| Institution: | Institute of Botany, Chinese Academy of Sciences, Guangzhou 510520. |
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| Abstract: | Total genomic DNA samples of Chinese traditional medicine(CTM) Cortex Magnoliae Officinalis ("Houpo") ,its counterfeits and substitutes were amplified by DNA amplification fingerprinting (DAT) .Their amplified DNA banding patterns were distinguished on PAGE.The results show that DAF can be used to identify CTM "Houpo",its counterfeits and substitutes, which is also quite valuable for correctly introducing original plant. |
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| Keywords: | Cortex Magnoliac Officinalis DAF Counterfeit Substitute |
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