骨关节炎软骨及软骨下骨吡啶并林含量的变化

目的：测定骨关节炎（ＯＡ）软骨及软骨下骨中呢啶并林（Ｐｙｒ）含量的变化。判断Ｐｙｒ在ＯＡ诊断和现活跃程度评价方面的作用。方法：复制成年兔膝关节ＯＡ模型，取有内髁主要负重区关节软骨及软骨下骨，采用高效液相色谱法（ＨＰＬＣ）测定其Ｐｙｒ含量。结果：８周内，关节软骨中Ｐｙｒ含量逐渐下降；软骨下骨中Ｐｙｒ含量在模型术后早期下降，随后逐渐升高。结论：ＯＡ关节软骨及软骨下骨的Ｐｙｒ含量随病程发生变化，可能是造

Pyridinoline Concentration Changes in Osteoarthritic Articular Cartilage and Subchondral Bone-An Experimental Study

ObjectiveThe urinary level of the pyridinoline(Pyr) had been shown to be elevated in Osteoarthritis(OA), but there were few studys which had focused on the concentration changes of the Pyr in OA cartilage and subchondral bone. The prupose of this study was to investigate the concentration changes of the Pyr in OA cartilage and subchondral bone, so as to assess the potential usefulness of the Pyr in OA diagnosing and disease activity evaluation. MethodsThe OA models of knee joint instibility in healthy adult rabbits were developed, then articular cartilage and subchondral bone in main load area of medial tibial condylus were harvested. Concentrations of Pyr were measured by high pressure liquid chromatography(HPLC) technique in cartilage and subchondral bone. ResultsPyr concentrations of cartilage decreased in the total study period of 8 weeks, while Pyr concentrations of subchondral bone decreased at first, and then it appeared an increasing tendency. ConclusionsConcentrations of Pyr in cartilage and subchondral bone changed with the progress of OA, which perhaps connected with the high excretion of Pyr in urine. Detecting changes of Pyr levels in urine has potential utility in OA diagnosing and disease activity evaluation.