钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究

目的 研究钛片表面粗糙度和氧化膜对成骨细胞黏附的影响,为选择临床种植体的表面处理方法提供依据.方法 250片纯钛钛片分为5组.采用直径分别为108～130 μm(S<,1>组)、216～301 μm(S<,2>组)、356～411 μm(S<,3>组)TiO<,2>砂对钛片表面进行喷砂处理:另外一组钛片采用钛浆喷涂(T...

Effect of surface roughness and titanium dioxide layers on commercially pure titanium on attachment of osteoblasts

Objective To study the effects of surface roughness and titanium dioxide（TiO2） layers on commercially pure titanium（cp-Ti） substrates on attachment of osteoblasts in vitro. Methods 250 pure titanium slices were divided into five groups. Osteoblasts were cultured on five cp-Ti substrates of ground, which blasted with 108-130 μm（S1）, 216-301 μm（S2）, 356-411 μm（S3） TiO2 particles and titanium-sprayed plasma（TPS） surfaces, surfaces prepared by hand grinding with SiC paper to 600 grits served as control（S0）. Surface average roughness and the TiO2 film structure was evaluated. For morphology and attachment measurement, osteoblasts were cultured for 1, 4, 12 and 24 h, evaluated by scanning electronic microscope（SEM） observation and MTT assay. Results Osteoblasts spread well on the titanium surfaces. Further more, osteoblasts spread more well on S3 surfaces. After 1 and 4 h culture, the number of cells on S3 surfaces was the highest（P<0.05）. The number of cells on S3 surfaces was the same（P>0.05） as TPS surfaces and higher than other groups（P<0.05） after 12 and 24 h. The number of cells of all experimental groups were higher than S0 surfaces after 4, 12 and 24 h（P<0.05）. Conclusion It was concluded that the coarse TiO2 particles blasted surface would optimize initial osteoblast responses.