急性白血病病人血浆TM、PC抗原和D-D测定及其临床意义

①目的　探讨急性白血病病人治疗前后血浆凝血酶调节蛋白 (TM)、蛋白C(PC)抗原、D 二聚体 (D D)含量的变化及其意义。②方法　用酶联免疫吸附测定方法对 12例健康体检者及 2 2例急性白血病病人治疗前后血浆TM、PC抗原和D D含量进行检测。③结果　急性白血病病人化疗前血浆TM、D D升高 ,PC抗原降低 ,与正常对照组比较有显著性差异 (t=3.5 0 9～ 6 .0 77,P <0 .0 1)。缓解后 ,血浆TM、PC抗原均恢复至正常水平 ,血浆D D含量比化疗前有明显降低 (t=5 .0 99,P <0 .0 1) ,但仍高于正常水平 (t=3.5 0 9,P <0 .0 1)。④结论　急性白血病病人存在不同程度的血管内皮细胞损伤及凝血和纤溶系统的激活 ,并随病情的好转而改善 ,血浆TM、PC抗原和D D含量可作为急性白血病病情进展、疗效观察及预后评估的指标之一

A DETECTION OF PLASMA THROMBOMODULIN,PROTEIN C ANTIGEN AND D-DIMER IN ACUTE LEUKEMIA AND ITS CLINICAL SIGNIFICANCE

Objective To study the changes of plasma thrombomodulin(TM), protein C(PC) antigen and D-Dimer(D-D) in patients with acute leukemia(AL) before and after treatment and their significance. Methods Twenty-two AL patients and 12 healthy controls were enrolled in the study. The levels of plasma TM, PC antigen and D-D were measured, before and after treatment, by enzyme-linked immunosorbent assay (ELISA). Results Before the treatment, the plasma levels of TM and D-D were higher while plasma PC antigen levels lower in AL patients compared with the normal controls, the difference being significant (t=3.509-6.077,P<0.01). After complete remission, the plasma levels of TM and PC antigen returned to normal, but those of D-D increased significantly compared with those of the normal controls (t=3.509,P<0.01). Conclusion Different degrees of activation of coagulation and fibrinolysis as well as injury of vascular endothelium exist in AL patients, and these will be improved with amelioration of the disease. The plasma levels of TM, PC antigen and D-D can be used as the indicators to predict the development, treatment outcome and prognosis of AL.