解脲脲原体对四环素类药物耐药性变化及机制研究

目的分析解脲脲原体(UU)对四环素类药物的耐药性变化,并探讨其耐药机制。方法取疑似泌尿生殖道感染患者的分泌物进行解脲脲原体培养,同时检测其对多西环素、米诺环素的敏感性;分离对上述两种药物耐药的UU菌株,采用PCR试剂盒检测四环素类耐药基因TetM。结果2003～2005年间共培养检测泌尿生殖道分泌物2845例,UU阳性1213例,3年间UU对多西环素、米诺环素的耐药性没有明显变化,2003年耐药率为5.6%和7.7%,2004年为5.4%和5.5%,2005年为8.2%和7.6%。同时对多西环素、米诺环素耐药的20株UU中均检出四环素类耐药基因TetM。结论四环素类药物仍然是治疗UU感染的敏感药物;TetM耐药基因是导致UU对四环素类药物耐药的主要机制。

Study of the Changes and Mechanism of the Ureaplasma Urealyticum Resisting to Tetracyclines

Objective To evaluate the changesand mechanisms of Ureaplasma urealyticum(UU) drug resistance to tetracyclines.Methods The secretion specimens collected from patients with the non-gonoccocal urethritis (NGU) were tested by the methods of incubating and drug susceptibility.The TetM genes were determined by PCR.Results There were no significant variance of the resistance to tetracyclines in Ureaplasma urealyticum (UU)during 2003-2005 years.The resistant rates of DOX, MIN were 5.6%and 7.7%(2003year),5.4%and 5.5%(2004 year),8.2%and 7.6%(2005 year),respectively.The positive rates of TetM genes in these samples resisting to DOX and MIN simultaneously were 100%(20/20).Conclusion The Tetracyclines are sensitivity drugs for UU yet;The TetM gene is the main cause for UU resisting to tetracyclines.