Effects of Lycium barbarum polysaccharides with different molecular weights on function of RAW264.7 macrophages |
| |
| Authors: | Xiangliang Deng Qi Liu Yajun Fu Xia Luo Minghua Hu Fangli Ma |
| |
| Institution: | 1. School of Pharmaceutical Sciences, Guangzhou Higher Education Mega Center, Guangzhou University of Chinese Medicine, Guangzhou, People’s Republic of China;2. Infinitus Chinese Herbal Immunity Research Centre, Guangzhou, People’s Republic of China;3. Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan, People’s Republic of China;4. Infinitus Chinese Herbal Immunity Research Centre, Guangzhou, People’s Republic of China |
| |
| Abstract: | The present study was aimed at investigating the effects of four LBP fractions with different molecular weights (MWs), designated LBP2, LBP3, LBP4 and LBP5, on RAW264.7 macrophages function. Results showed that LBP fractions could significantly enhance the expression of CD86 and MHC-II molecules on RAW264.7 macrophages. LBP3, LBP4 and LBP5 could enhance the production of ROS, NO, TNF-α and IL-6, and the phagocytosis of RAW264.7 macrophages. LBP2 with an MW of larger than 350?kDa could only enhance the secretion of TNF-α. LBP3 enhanced the RAW264.7 macrophages function in a dose-dependent manner and also enhanced the iNOS mRNA expression in the cells. These results demonstrated that the immunomodulatory activity of LBP on RAW264.7 macrophages was closely related to its MW. It indicated that fractions with an MW smaller than 350?kDa were the main active fractions of LBP in enhancing macrophages function. |
| |
| Keywords: | Lycium barbarum polysaccharide molecular weight macrophages Griess reagent flow cytometer |
|
|
