Immunochromatographic strip for ultrasensitive detection of fumonisin B1 |
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| Authors: | Kai Hao Steven Suryoprabowo Tao Hong Shanshan Song Liqiang Liu Qiankun Zheng |
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| Affiliation: | 1. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, People's Republic of China;2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China;3. International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China;4. National Institute of Metrology, Beijing, People's Republic of China;5. Delishi Group, Weifang, Shandong, People's Republic of China |
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| Abstract: | A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50?ng/mL in 0.01?M PBS and 25?ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1. |
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| Keywords: | Fumonisins Fumonisin B1 immunochromatographic assay monoclonal antibody strip test |
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