Steroid-depleted polycystic ovarian syndrome serum promotes in vitro oocyte maturation and embryo development |
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| Authors: | Elham Eyvaznejad Mohammad Nouri Aliyeh Ghasemzadeh Vahideh Shahnazi Samira Asghari |
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| Affiliation: | 1. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran;2. Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran;3. Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran |
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| Abstract: | In vitro maturation (IVM) of immature oocytes obtained from patients with polycystic ovarian syndrome (PCOS) is considered as a novel strategy in order to reduce clinical side effects and cost of in vitro fertilization (IVF) technique. The aim of this study was to evaluate the effects of PCOS whole and steroid-depleted serums on in vitro oocyte maturation indices. Patients with PCOS were selected according to the Rotterdam criteria. Cumulus–oocyte complexes and blood serums were collected and pooled. Cumulus cells and immature oocytes were treated with 10% whole or steroid-depleted serums. Stearoyl-CoA desaturase-1 (SCD1) and cyclooxygenase-2 (COX2) expression levels in cumulus cells were evaluated by quantitative PCR. Fatty acid composition of cumulus cells was analyzed using gas–liquid chromatography. Polar body observation was considered as the oocyte maturation index. Oleate (1.28-fold, p?=?.006), SCD1 expression (450-fold, p?=?.001), and COX2 expression (35-fold, p?=?.02) in cumulus cell, as well as oocyte maturation (p?in vitro embryo development (p?
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| Keywords: | COX2 cumulus cells IVM PCOS SCD1 |
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