| 纳米材料复合人骨髓成骨细胞培养的研究 |
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| 引用本文： | 苗宗宁,戴涟生,祝建中,王玲,陆华,惠国桢.纳米材料复合人骨髓成骨细胞培养的研究[J].实用临床医药杂志,2003,7(3):212-214. |
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| 作者姓名： | 苗宗宁 戴涟生 祝建中 王玲 陆华 惠国桢 |
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| 作者单位： | 1. 江苏省无锡市第三人民医院细胞实验室,江苏,无锡,214001
2. 苏州大学附属第一医院,江苏苏州,215007 |
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| 基金项目： | 无锡市社会发展项目(CS2 0 0 2 0 0 0 4) |
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| 摘 要： | 目的 探讨纳米材料作为组织工程骨基质材料的可行性。方法 分离培养人骨髓间充质干细胞 ,诱导为成骨细胞后作为种子细胞 ,与纳米晶羟基磷灰石胶原材料体外联合培养 ,通过对复合物光镜及扫描电镜观察 ,了解细胞在材料中生长情况。结果 人骨髓间充质干细胞可以诱导为成骨细胞 ,体外复合培养 2周 ,分布于支架材料上的细胞大量分化增殖。结论 纳米晶羟基磷灰石胶原材料是一种构建组织工程骨的较好的支架材料
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| 关 键 词： | 纳米材料 骨髓间充质干细胞 成骨细胞 组织工程 |
| 文章编号： | 1672-2353(2003)03-0212-03 |
| 修稿时间： | 2003年3月8日 |
EXPERIMENTAL STUDY OF OSTEOBLASTS FROM HUMAN BONE MARROW COCULTURE WITH THE NANOMETER MATERIAL |
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| MIAO Zong-ning ,DAI Lian-sheng ,ZHU Jian-zhong ,WANG Ling ,LU Hua ,HUN Guo-zhen.EXPERIMENTAL STUDY OF OSTEOBLASTS FROM HUMAN BONE MARROW COCULTURE WITH THE NANOMETER MATERIAL[J].Journal of Clinical Medicine in Practice,2003,7(3):212-214. |
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| Authors: | MIAO Zong-ning DAI Lian-sheng ZHU Jian-zhong WANG Ling LU Hua HUN Guo-zhen |
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| Institution: | MIAO Zong-ning 1,DAI Lian-sheng 1,ZHU Jian-zhong 1,WANG Ling 1,LU Hua 1,HUN Guo-zhen 2 |
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| Abstract: | Objective: To investigate the feasibility of the nanometer crystal hydroxyapatite collagen material as scaffold material in bone tissue engineering. Methods: Osteoblasts, which were induced from mesenchymal stem cells isolated from human bone marrow, were cultured as the seeding cells, then the cells were cocultured with the nanometer crystal hydroxyapatite collagen material in vitro. The cell-material complex was observed under lightmicroscope and electronic scanning microscope in order to evaluate the interacting between cells and material. Results: It is possible to induce mesenchymal stem cells, isolated from bone marrow, into osteoblasts. Two weeks after coculture, the osteoblasts differentiated and proliferated in the nanometer crystal hydroxyapatite collagen material network. Conclusion: The nanometer crystal hydroxyapatite collagen material is a good scaffold material for the remodeling bone in bone tissue engineering. |
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| Keywords: | nanometer material mesenchymal stem cells osteoblasts tissue engineering |
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