重组人骨形态发生蛋白2在下颌骨牵引成骨过程中的作用

背景：重组人骨形态发生蛋白2能诱导未分化的间充质细胞不可逆地分化形成软骨和骨，从而导致新骨的形成。目的：观察在下颌骨牵引成骨过程中应用基因重组人骨形态发生蛋白2(rhBMP-2)对成骨的影响。方法：选用日本成年大耳白兔45只，随机分为3组，建立下颌骨牵引模型，分别将空白胶原、1.5 mg和3.0 mg重组人骨形态发生蛋白2胶原复合物植入下颌骨切开处，固定、牵引0.4 mm/次，2次/d，共计5 d，总延长下颌骨4 mm。在稳定期第1，3，7，14，28天分别处死各组动物，取牵引区新生骨痂行放射学及组织学检测。结果与结论：重组人骨形态发生蛋白2组中的新生骨组织较空白胶原对照组中多且成熟，3.0 mg重组人骨形态发生蛋白2组较1.5 mg重组人骨形态发生蛋白2组新骨形成多且成熟。结果表明，重组人骨形态发生蛋白2能促进兔下颌骨牵引成骨，并且具有浓度依赖性。

Effects of recombinant human bone morphogenetic protein 2 on mandibular distraction

BACKGROUND: Recombinant human bone morphogenetic protein 2 (rhBMP-2) can induce undifferentiated mesenchymal cells irreversibly differentiated into cartilage and bone, thus, lead to the formation of new bone.OBJECTIVE: To study the effect of rhBMP-2 on the mandibular distraction.METHODS: A total of 45 Japanese white rabbits were prepared for mandibular distraction models and randomly assigned into control, 1.5 mg rhBMP-2 and 3.0 mg rhBMP-2 groups. Collagen sponge and collagen binding proteins were implanted into mandibles of rabbits, respectively, and distraction 0.4 mm per time, twice per day for 5 days. The mandibles were lengthened 4 mm. All rats were sacrificed at 1, 3, 7, 14 and 28 days after operation, and the new generated bone were detected by X-ray and histological examination. RESULTS AND CONCLUSION: Compared with the control group, there were more neonatal bone tissues with maturity in the rhBMP-2 group, especially in the rhBMP-2 3.0 mg group. The results demonstrated that RhBMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in a concentration-dependent manner.