| EBV转化的人B淋巴母细胞和人B淋巴细胞杂交瘤细胞表面标记表达的研究 |
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| 引用本文： | 朱勇,金伯泉,赵宁,刘雪松,黄传书. EBV转化的人B淋巴母细胞和人B淋巴细胞杂交瘤细胞表面标记表达的研究[J]. 细胞与分子免疫学杂志, 1995, 0(2) |
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| 作者姓名： | 朱勇 金伯泉 赵宁 刘雪松 黄传书 |
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| 作者单位： | 第四军医大学免疫学教研室 |
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| 摘 要： | 本文用碱性磷酸酶—抗碱性磷酸酶（ＡＰＡＡＰ）免疫组化染色技术，对ＥＢ病毒（ＥＢＶ）转化的人Ｂ淋巴母细胞（ＬＣＬ）、人Ｂ淋巴细胞杂交瘤以及它们的亲本骨髓瘤细胞系的部分表面标记进行了检测。结果显示，人Ｂ淋巴细胞在ＥＢＶ转化或杂交过程中表面标记发生了较大的变化；对ＬＣＬ和杂交瘤细胞表面标记的检测可能为探寻人Ｂ细胞在这些过程中染色体保留或丢失的规律提供一种手段。此外，ＬＣＬ及人Ｂ细胞杂交瘤细胞系有可能被用作一种新的模型，来研究人Ｂ淋巴细胞的分化和功能。
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| 关 键 词： | 表面标记；人B细胞杂交瘤；APAAP |
Analysis of The Expression of Surface Markers on Human LCLs and Human B-Lymphocyte Hybridoma Cell Lines |
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| Zhu Yong,Jin Boquan,Zhao Ning,Liu Xuesong and Hang Chuanshu. Analysis of The Expression of Surface Markers on Human LCLs and Human B-Lymphocyte Hybridoma Cell Lines[J]. Chinese journal of cellular and molecular immunology, 1995, 0(2) |
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| Authors: | Zhu Yong Jin Boquan Zhao Ning Liu Xuesong Hang Chuanshu |
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| Abstract: | Here we analyzed the expression of some surface markers on EB virus(EBV)transformedB lymphoblastoid cells(LCLs),human B-lymphocyte hybridoma cell lines and their parent myelomacell lines using alkaline phosphatase-anti-alkaline phosphatase(APAAP)immunocytochemistry technique.The results demonstrated that the surface markers on human B lymphocytes changed a lot afterEBV transformation or hybridization.Thus,analysis of surface markers could be useful for exploringthe nature of chromosomes retained or lost in human B lymphocytes during the processes above.Moreover,LCLs and human B-lymphocyte hybridoma cell lines probably were potential modals for studyingdifferentiation and functions of human B lymphocytes. |
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| Keywords: | surface marker human B-lymphocyte hybridoma APAAP |
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