| 贝母素乙对放射性肺损伤大鼠肺组织水通道蛋白的影响 |
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| 引用本文： | 吉福志,何栋成,龚婕宁,赵晓峰,郭海.贝母素乙对放射性肺损伤大鼠肺组织水通道蛋白的影响[J].医学研究杂志,2015,44(12):41-44. |
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| 作者姓名： | 吉福志 何栋成 龚婕宁 赵晓峰 郭海 |
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| 作者单位： | 223300 南京医科大学附属淮安第一医院放疗科;223300 南京医科大学附属淮安第一医院放疗科;210023 南京中医药大学;223300 南京医科大学附属淮安第一医院中医科;223300 南京医科大学附属淮安第一医院中医科 |
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| 基金项目： | 国家自然科学基金资助项目(81373603);江苏省博士后科研资助计划第2批基金资助项目(1102170C);南京医科大学科技发展基金资助项目(2012NJMU153) |
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| 摘 要： | 目的研究贝母素乙对肺损伤大鼠水通道蛋白AQP-1、AQP-5的影响,阐明贝母素乙治疗肺损伤的机制。方法将大鼠采用数字法随机分为4组:正常组(蒸馏水1ml/100g体重)10只,模型组(蒸馏水1ml/100g体重)12只、地塞米松组(等容积地塞米松蒸馏水溶液)12只、贝母素乙组(等容积贝母乙素蒸馏水溶液)12只,每组雌雄各半。6MV高能X线直线加速器15Gy/(3F·3W)照射,建立大鼠全肺放射性肺损伤模型。肺组织行HE染色,观察肺泡炎症程度。采用放射免疫法检测放射性肺损伤大鼠肺组织中AQP-1、AQP-5含量,荧光定量RT-PCR分析AQP-1、AQP-5 mRNA表达。结果贝母素乙可增高放射性肺损伤大鼠肺组织AQP-1、AQP-5水平,调节AQP-1、AQP-5 mRNA表达。结论贝母素乙具有调节AQP-1、AQP-5的作用,这可能是治疗放射性肺损伤作用机制的关键。
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| 关 键 词： | 贝母素乙 水通道蛋白 放射性 肺损伤 |
| 收稿时间： | 2015/3/26 0:00:00 |
| 修稿时间： | 2015/4/23 0:00:00 |
Effect of Peiminine on Aquaporins of Lunl Injury in Rats. |
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| Ji Fuzhi,He Dongcheng,Gong Jiening.Effect of Peiminine on Aquaporins of Lunl Injury in Rats.[J].Journal of Medical Research,2015,44(12):41-44. |
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| Authors: | Ji Fuzhi He Dongcheng Gong Jiening |
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| Institution: | Huaian First Peoples Hospital, Nanjing Medical University, Nanjing University of Chinese Medicine, Jiangsu 223300, China;Huaian First Peoples Hospital, Nanjing Medical University, Nanjing University of Chinese Medicine, Jiangsu 223300, China |
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| Abstract: | Objective To study the effect of Peiminine on AQP-1 and AQP-5 against radiation-induced lung injury in rats and clarify its mechanism of treatment. Methods A total of 46 SD healthy rats were randomly assigned into 4 groups,including the comparison group,the radiation group,the dexamethaane group and the Peiminine group.The model in rats was established by irradiating whole rats chest with the dose of 15 Gy/3F of 6 MV X-rays.The activities of AQP-1 and AQP-5 in lung tissues were examined by immunoradiometric.AQP-1, AQP-5 expressions in lung tissues were detected by immunohistochemistry and RT-PCR. Results The results in our study indicated that peiminine had beneficial effects to protect against radiation-induced lung injury in rats.The attenuation of pulmonary fibrosis by peiminine was associated with a increasing in the levels of AQP-1, AQP-5, regulation of AQP-1, AQP-5 mRNA expression in lung tissue. Conclusion Our results thus provide evidence that peiminine have therapeutic potential for the treatment pulmonary fibrosis. |
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| Keywords: | Peiminine Aquaporin Radioaction Lung injury |
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