绞股蓝总皂苷对阿霉素致大鼠急性心脏毒性的保护作用

目的: 观察绞股蓝总皂苷(gypenosides,Gps)对阿霉素致急性大鼠心脏毒性的保护作用,探讨其作用机制。方法: 雄性SD鼠24只,随机分为正常对照组(normal control,NC)、阿霉素组(adriamycin,ADR)和绞股蓝总皂苷组(Gps)。第3组给予4%绞股蓝总皂苷400mg·kg-1·d-1,分早晚2次灌胃,其它两组灌注等量生理盐水,连续5天。第6天一次性腹腔注射阿霉素10mg/kg复制大鼠急性心脏毒性模型,利用PowerLab数据采集和分析系统采集心室内压并分析心功能,检测心肌组织超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)的含量。结果: 与NC组相比,阿霉素组左室收缩峰压(left ventricular systolic pressure,LVSP)、左室内压最大上升/下降速率(maximal rates of pressure development anddecline,±dp/dt_max)、SOD的活性均显著降低(P<0.01),MDA含量显著升高(P<0.01)。与ADR组相比,Gps组的LVSP、±dp/dt_max和SOD均显著升高(P<0.01),MDA下降(P<0.05)。结论: 绞股蓝总皂苷可减轻阿霉素对心脏的毒性作用,明显改善大鼠的心功能。

Protective effect of gypenosides on adriamycin induced acute cardiotoxicity in rats

Objective:To observe the protective effect of gypenosides(Gps)on adriamycin(ADR)induced acute cardiotoxicity in rats.Methods:Rats in Gps group received gypenosides 200 mg/kg orally twice a day for five days.In the meanwhile,the saline was given to ADR group and normal rats as controls.At the sixth day,the acute heart toxicity was induced by intraperifoneal injecting ADR(10 mg/kg).Subsequently,the functions of cardiac muscle,such as left ventricular systolic pressure(LVSP)and left verfricle maximal rates of pressure development and decline(+dp/dtmax),as well as the activity of SOD and content of MDA were measured.Results:LVSP,+dp/dtmax and the activity of SOD in rats of ADR group were significantly lower,and the content of MDA was higher than those in controls(P<0.01).LVSP,+dp/dtmaxand the activity of SOD in rats of Gps group were significantly higher and the content of MDA was significantly lower than those without giving gypenosides(P<0.05-P<0.01).Conclusions:Gyp secure cardiac muscles and their function from adriamycin impairment.