| 中性粒细胞产生活性氧的四种测定方法 |
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| 引用本文： | 胡明昌,姜新猷. 中性粒细胞产生活性氧的四种测定方法[J]. 南京医科大学学报(自然科学版), 1993, 0(4) |
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| 作者姓名： | 胡明昌 姜新猷 |
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| 作者单位： | 南京医学院第二附属医院儿科(胡明昌)，南京医学院第二附属医院儿科(姜新猷) |
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| 摘 要： | 分别介绍了高铁细胞色素C还原法,四唑氮蓝还原法测定中性粒细胞产生O_2~-7·和辣根过氧化物酶酚红,氨基安替比林-酚红法测定中性粒细胞产生H_2O_2。研究表明,高铁细胞色素C还原法和氨基安替比林-酚红法测定活性氧灵敏性高,特异性强。
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| 关 键 词： | 中性粒细胞 呼吸链 活性氧检测 |
An Evaluation on Methods for Measurement of Reactive Oxygen Species from Neutrophils |
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| Hu Mingchang,Jiang Xinyou. An Evaluation on Methods for Measurement of Reactive Oxygen Species from Neutrophils[J]. Acta Universitatis Medicinalis Nanjing, 1993, 0(4) |
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| Authors: | Hu Mingchang Jiang Xinyou |
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| Affiliation: | Hu Mingchang,Jiang Xinyou Department of Pediatrics,the Second Affiliated Hospital of Nanjing Medical College,Nanjing |
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| Abstract: | A comparison was made on the relative merits of four methods for the mea-suration of reactive oxygen species from neutrophils: Ferric cytochrome c reduction assayand nitroblue tetrazolium reduction assay for determination of superoxide anion, horseradishperoxidase-phenol red assay and phenol red-aminoantipyrine assay for quantitation of hydro-gen peroxide. The results revealed that ferric cytochrome c reduction assay and phenol red-aminoantipyrine assay are more specific and sensitive than the other two methods. |
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| Keywords: | neutrophil reactive oxygen species measurement respiratory chain |
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