系统性红斑狼疮患者外周血T淋巴细胞亚群、免疫球蛋白及补体变化分析

【摘要】目的探讨T淋巴细胞亚群、免疫球蛋白及补体在系统性红斑狼疮（SLE）患者中的变化及临床意义。方法对55例SLE患者外周血CD3＋、CD4＋、CD8＋T淋巴细胞、免疫球蛋白及补体进行检测分析，另选取50名健康体检者作为对照组。CD3＋、CD4＋、CD8＋T淋巴细胞用流式细胞仪检测，免疫球蛋白及补体采用透射比浊法在全自动生化仪上检测。结果研究组外周血CD3＋、CD4＋、CD8＋T淋巴细胞绝对值及CD4＋／CD8＋比值、C3、C4明显低于对照组，IgG、IgA、IgM明显高于对照组（P〈0．01）。结论SLE患者T淋巴细胞亚群、免疫球蛋白及补体水平变化明显，三者联合检测，有利于病情监测及指导治疗。

Study on changes in peripheral blood T lymphocyte subsets, immunoglobulin and complement in SLE patients.

Objective To explore the changes and clinical significance of T lymphocyte subsets, immunoglobulin, and complements in patients with systemic lupus erythematosus （SLE）. Methods The levels of peripheral blood CD3 ＋ CD4 ＋ and CDs ＋ T lymphocytes, immunoglobulin and complements in 55 cases of SLE were detected and analyzed. Fifty healthy persons were allocated in control group. CD3, ＋ CD4 ＋ and CD8 ＋ T lymphocytes were detected by flow cytometry, immunoglobulin and complements were detected with turbidimetrie method by automatic biochemical analyzer. Results In comparison with normal control group, the absolute number of CD3 ＋ CD4 ＋ and CDs ＋ lymphocytes and CD4 ＋ / CD8＋ ratio, C3 and C4 in study group were significantly decreased （ P 〈 0.01 ） , blood levels of IgG, IgA and IgM were significantly increased （ P 〈 0.01 ）. Conclusion The levels of T lymphocyte subsets, immunoglobulin and complements in patients with SLE were significantly changed. The joint detection of 3 markers has important clinical significance in comprehensive understanding of the immune status of host and correct evaluation of the Severity of disease, thus it may guide the clinical treatment.