亚剂量吡喹酮对慢性血吸虫病患者循环抗原水平的影响

目的观察不同亚治疗剂量吡喹酮对慢性血吸虫病患者血清血吸虫肠相关趋阴极抗原（ＧＡＣＡ）水平的影响。方法ＫａｔｏＫａｔｚ法粪检确定１０８例慢性血吸虫病患者，随机分成４组，分别给予口服５ｍｇ／ｋｇ、１０ｍｇ／ｋｇ、２０ｍｇ／ｋｇ剂量吡喹酮和安慰剂。采用单克隆抗体酶联免疫吸附试验（ＭｃＡｂＤｏｔＥＬＩＳＡ）分别检测服药前、后２、８、２４和４８小时患者血清ＧＡＣＡ水平。服药前、后检出率变化采用χ２检验。结果３个实验组服药后２小时检测血清ＧＡＣＡ水平，平均较服药前上升３个滴度，后渐下降；至服药后４８小时，血清ＧＡＣＡ水平仍高于服药前１个滴度。血清ＧＡＣＡ检测敏感性，可由服药前的８２．７％提高到服药后２小时的１００．０％（Ｐ＜０．０５）。对照组４８小时观察期内，血清ＧＡＣＡ水平波动在１个滴度范围内，检测敏感性无明显变化（Ｐ＞０．０５）。结论口服不同亚治疗剂量吡喹酮均能有效升高慢性血吸虫病患者血清血吸虫ＧＡＣＡ水平，并显著提高血清ＧＡＣＡ检测敏感性。

Effect of suabclinical doses of praziquantel on circulating antigen levels in the patients with chronic Schistosomiasis

Objective To observe the effect of different subclinical doses of praziquantel on the serum gutassociated cathodic antigen (GACA) levels in the patients with chronic schistosomiasis. Methods One hundred and eight cases of patients with chronic schistosomiasis diagnosed by KatoKatz method were divided into 4 groups,which were given praziquantel at the dosage of 5mg/kg,10mg/kg,20mg/kg and placebo respectively.The serum samples were collected and detected by McAbDotELISA before treatment and at 2h,8h,24h and 48h after treatment.Statistic analysis was 2 test. Results The serum GACA levels of all three experimental groups at 2h after treatment were higher than that before treatment by 3 titers.However,the titer was decreased gradually after 2h of the thereapy. Till 48h after treatment the serum GACA levels were still 1 titer higher than that before treatment. The positive detection rate of serum GACA was raised from 82.7% before treatment to 100.0% at 2h after treatment (P<0.05).Comparatively,there were no obvious changes on the serum GACA levels and the positive detection rate in the control group (P>0.05) during the same period of observation. Conclusion Different subclinical doses of praziquantel can increase the serum GACA levels as well as the sensitivity of GACA detection effectively in the patients with chronic schistosomiasis significantly.