口服丙型肝炎复合多表位DNA减毒鼠伤寒沙门活菌苗的实验研究

目的　利用减毒鼠伤寒沙门菌作为载体，探讨丙型肝炎病毒（ＨＣＶ） 口服ＤＮＡ 疫苗的可行性。方法　把ＨＣＶ 复合多表位抗原基因ＰＣＸ 克隆到真核表达载体ｐｃＤＮＡ３（ＣＭＶ 启动子） ，构建ＨＣＶ 真核表达载体ｐｃＤＮＡ３／ＰＣＸ，转化减毒鼠伤寒沙门菌ＳＬ３２６１ ，获得ＨＣＶ 重组口服ＤＮＡ 活菌苗ＳＬ３２６１ （ｐｃＤＮＡ３／ＰＣＸ） ，免疫小鼠及家兔后，检测特异性免疫应答及安全性。结果　ＳＬ３２６１（ｐｃＤＮＡ３／ＰＣＸ） 在小鼠及家兔中均可诱发低水平的特异性体液免疫及细胞免疫应答，免疫动物未见明显的毒性反应。结论　ＨＣＶ 口服减毒鼠伤寒沙门菌ＤＮＡ 疫苗可诱发特异性的免疫应答，为ＨＣＶ 疫苗的研究提供新的理论及实验依据

Immunogenicity of a multi-epitopes antigen gene of hepatitis C virus carried by attenuated Salmonella typhimurium SL3261

Objective To use Salmonella typhimurium as a vehicle to explore the possibility of oral live DNA vaccine against hepatitis C virus (HCV).Methods A multi epitopes antigen gene of HCV was cloned into a eukaryotic vector pcDNA3 and introduced into attenuated Salmonella typhimurium SL3261 to construct HCV oral live DNA vaccine candidate SL3261 (pcDNA3/PCX). The recombinant bacterium was used to orally immunize mice and rabbits. Meanwhile, specific humoral and cellular immune responses and safety were detected.Results Low level of specific antibodies and cellular immune responses were induced without obvious toxicity in the immunized mice and rabbits after oral administration of the live bacteria.Conclusion The results indicate that the HCV recombinant oral live DNA vaccine can induce specific immune responses which might be able to provide an alternative for HCV vaccines.