寻常性银屑病皮损处神经纤维的数量及其与朗格汉斯细胞关系的观察

目的 观察寻常性银屑病患者皮损处神经纤维的数量变化及其与朗格汉斯细胞的关系.方法采用免疫组化过氧化物酶法观察28例寻常性银屑病患者皮损处神经纤维的数量变化;采用免疫荧光双标记及共聚焦激光扫描显微镜技术观察寻常性银屑病患者皮损处神经纤维与朗格汉斯细胞的关系.结果寻常性银屑病患者皮损处神经纤维长度显著增加,与正常人对照比较有统计学差异(t=4.09,P＜0.001).皮损表皮内神经纤维与朗格汉斯细胞的接触明显增多,与正常人对照比较有统计学差异(t=3.55,P＜0.01).结论寻常性银屑病皮损处神经纤维长度显著增加,表明此部位神经纤维增生明显,并且表皮内神经纤维与朗格汉斯细胞的直接接触明显增加,提示银屑病患者神经系统的免疫调控作用可能是通过改变朗格汉斯细胞的功能实现的。

Studies on Nerve Fibers and Their Relationship with Langerhans Cells in the Lesional Skin of Psoriasis Vulgaris

Objectives To observe and quantify the intraepidermal and papillary dermal nerve fibers,and the contact between intraepidermal nerve fibers and Langerhans cells in the lesional skin of pso-riasis vulgaris.Methods The nerve fibers and Langerhans cells were analyzed with immunohistochemical LAB-SA method,double-labelled immunofluorescence and confocal laser scanning microscopy in28biopsies of lesional skin taken from psoriatic patients and17normal controls.Results The length of nerve fibers was significantly longer in psoriatic lesions than that in normal controls(t =4.09,P<0.001).The quantity of contact between intraepidermal nerve fibers and Langerhans cells was significantly increased in psoriatic lesions than that in normal control(t =3.55,P <0.01).Conclusions The increased contact between in-traepidermal nerves and Langerhans cells in psoriatic lesions indicates that the modulatory effects of nervous system upon skin immune system might be fulfilled potentially by altering the functions of Langerhans cells.