| 人卵巢癌靶向超声造影剂的制备及其体外寻靶能力观察 |
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| 引用本文： | 贺娟,孙江川,常淑芳,李潘,王志刚.人卵巢癌靶向超声造影剂的制备及其体外寻靶能力观察[J].中国医学影像技术,2009,25(6):929-931. |
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| 作者姓名： | 贺娟 孙江川 常淑芳 李潘 王志刚 |
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| 作者单位： | 1. 重庆医科大学附属第二医院妇产科,重庆,400010
2. 重庆医科大学附属第二医院超声影像研究所,重庆,400010 |
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| 基金项目： | 863科技攻关项目，国家自然科学基金，重庆市卫生局项目 |
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| 摘 要： | 目的 制备人卵巢癌细胞靶向超声造影剂(TUCA),对其进行鉴定,观察其体外寻靶能力.方法 采用共价结合法制备TUCA即黄体生成素释放激素-脂质体微泡(LHRH-LM):免疫荧光染色试验证明配体LHRH与LM的结合,倒置显微镜下观察TUCA与人卵巢癌细胞株OVCAR-3的结合情况,观察LHRH-LM对OVCAR-3细胞的体外寻靶能力;同时以LM为对照,并用LHRH做阻断试验.结果 LHRH-LM的荧光免疫染色试验阳性;体外寻靶试验显示携带配体LHRH的LM能较好的黏附在OVCAR-3细胞上,经反复洗涤不掉,而作为对照的LM与OVCAR-3细胞株混合反应后经反复洗涤,微泡与细胞完全分离,无结合;LHRH成功阻断TUCA与癌细胞的结合.结论 采用共价结合法成功制备了靶向超声造影剂,该造影剂在体外能高效地与人卵巢癌细胞株OVCAR-3结合.
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| 关 键 词： | 靶向脂质微泡 卵巢肿瘤 黄体生成素释放激素 |
| 收稿时间： | 2008/12/23 0:00:00 |
| 修稿时间： | 2009/2/11 0:00:00 |
Preparation of targeted liposome microbubbles and observation of its targeted ability in human ovarian cancer in vitro |
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| HE Juan,SUN Jiang-chuan,CHANG Shu-fang,LI Pan and WANG Zhi-gang.Preparation of targeted liposome microbubbles and observation of its targeted ability in human ovarian cancer in vitro[J].Chinese Journal of Medical Imaging Technology,2009,25(6):929-931. |
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| Authors: | HE Juan SUN Jiang-chuan CHANG Shu-fang LI Pan and WANG Zhi-gang |
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| Institution: | Department of Gynaecology and Obstetrics, the Second Affiliated Hospital Chongqing Medical University, Chongqing 400010, China;Department of Gynaecology and Obstetrics, the Second Affiliated Hospital Chongqing Medical University, Chongqing 400010, China;Department of Gynaecology and Obstetrics, the Second Affiliated Hospital Chongqing Medical University, Chongqing 400010, China;Institute of Ultrasound Imaging, the Second Affiliated Hospital Chongqing Medical University, Chongqing 400010, China;Institute of Ultrasound Imaging, the Second Affiliated Hospital Chongqing Medical University, Chongqing 400010, China |
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| Abstract: | |
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| Keywords: | Targeted liposome microbubbles Ovarian neoplasms Leutenizing hormone releasing hormone |
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