| 胃癌中p16基因缺失与突变的研究 |
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| 引用本文： | 魏国 方国恩 毕建威 申晓军 华积德. 胃癌中p16基因缺失与突变的研究[J]. 临床肿瘤学杂志, 2005, 10(5): 455-458 |
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| 作者姓名： | 魏国 方国恩 毕建威 申晓军 华积德 |
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| 作者单位： | 第二军医大学长海医院普外科,上海200433 |
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| 摘 要： | 目的：探讨p16基因外显子2的缺失和突变与胃癌发生发展的关系.方法：应用新鲜组织标本基因组DNA抽提、PCR-SSCP分析的方法,对30例胃癌及癌旁组织中p16基因外显子2的缺失和突变进行检测.结果：胃癌组织样本中p16基因外显子2的缺失率为10.00%,突变率为10.00%,两者之和为20.00%,癌旁组织样本中未发现缺失和突变,统计学分析有显著性差异（P〈0.01）.结论：p16基因外显子2的缺失和突变与胃癌的发生具有一定的相关性.
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| 关 键 词： | 胃肿瘤 p16基因 PCR-SSCP 突变 |
| 文章编号： | 1009-0460(2005)05-0455-04 |
| 收稿时间： | 2004-12-25 |
| 修稿时间： | 2005-03-05 |
Deletion and mutation of p16 gene in primary gastric carcinoma |
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| WEI Guo,FANG Guo-en,BI Jian-wei,SHEN Xiao-jun,HUA Ji-de.. Deletion and mutation of p16 gene in primary gastric carcinoma[J]. Chinese Clinical Oncology, 2005, 10(5): 455-458 |
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| Authors: | WEI Guo FANG Guo-en BI Jian-wei SHEN Xiao-jun HUA Ji-de. |
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| Affiliation: | Department of General Surgery, Chang Hal Hospital, Shanghai 200433, China |
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| Abstract: | Objective:To study the relationship between the deletion and mutation of p16 gene in exon 2 and the occurrence of primary gastric carcinoma.Methods:Using molecular biological methods that include genome DNA extraction from fresh tissue and PCR-SSCP analysis technique,it was studied that the deletion and mutation of p16 gene in exon 2 in 30 cases of primary gastric carcinomas.Results:The total alteration rate of p16 gene in exon 2 in primary gastric carcinomas was 20.00%(6/30) in which the deletion rate was 10.00%(3/30) and the mutation rate was 10.00%(3/30).In contrast,neither deletions nor mutations was found in the control group of adjacent gastric mucosa,respectively with significant difference(P<0.01).Conclusion:The deletion and mutation of p16 gene in exon 2 is related to the genesis of primary gastric carcinoma. |
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| Keywords: | Gastric carcinoma p16 gene PCR-SSCP analysis Mutation |
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