HPM辐射后大鼠海马组织中细胞凋亡及Caspase 3的表达研究

目的探讨HPM辐射后大鼠海马组织中细胞凋亡及Caspase 3的表达及其意义.方法采用12 mW/cm2HPM模拟源辐照50只Wistar雄性大鼠,于照后6 h,1、3、7、14和28 d活杀取海马组织,经光镜、电镜、免疫组化和图像分析等技术,研究HPM辐射后大鼠海马组织中细胞凋亡的特点、Caspase 3的表达及其意义.结果12 mW/cm2HPM辐射后6 h～3 d,凋亡的细胞进行性增多,3 d达高峰,7 d后逐渐减少,14 d后恢复至正常水平.凋亡的细胞核染色质浓缩、边集.照射后1～3 d,Caspase-3于神经元胞浆中表达进行性增加,3 d达高峰,7 d后逐渐恢复,28 d基本恢复至正常水平.结论细胞凋亡是HPM辐射引起海马神经元死亡的主要方式,Caspase-3表达增加,参与HPM辐射后海马神经元凋亡的过程.

The study on apoptosis of neurons and expression of caspase-3 in rat hippocampus after high power microwave radiation

ObjectiveTo investigate the apoptosis of neurons and expression of caspase-3 in rat hippocampus after high power microwave(HPM) radiation.MethodsHPM device of mean power density(12 mW/cm~2) was used to radiate 50 healthy Wistar male rats.Light microscopy, transmission electron microscopy,immunohistochemistry and image analysis were used to study the rule of neuron apoptosis and the expression of caspase-3.ResultsThe apoptotic cells in hippocampus were increased on 6 h,peaked on(3 d),decreased on 7 d and recovered on 14 d after 12 mW/cm~2 HPM radiation,which the characteristics of apoptotic cells were the chromatin condensation and margination.Caspase-3 was increased in the cytoplasm of neurons in hippocampus on 1 d, peaked on 3 d and recovered on 7 d after radiation.ConclusionApoptosis is the main cell death of hippocampus neuron induced by HPM radiation.The expression of caspase-3 was increased significantly and played an important role in the course of apoptosis of hippocampus neuron induced by HPM radiation.