低剂量辐射对LAK/TIL培养扩增及抗肿瘤作用的实验研究

目的　为了解低剂量电离辐射对LAK/TIL细胞培养扩增及抗肿瘤作用的影响。方法　从荷瘤小鼠的脾脏制得LAK细胞 ,从荷Lewis肺癌小鼠瘤组织中分离得TIL细胞 ,给予不同剂量的X射线照射后培养。结果　低剂量电离辐射可增加LAK/TIL细胞的扩增量 ,降低细胞扩增过程死亡率 ,并增加细胞毒性。结论　低剂量电离辐射对生物活性细胞不仅有诱导扩增作用 ,同时亦降低扩增过程细胞死亡率。

Influence of low dose ionizing radiation on amplification and antitumor activity of LAK/TIL cells

Objective To study the influence of low dose ionization on amplification and antitumor activity of LAK/TIL cells. Methods TIL cells isolated from Lewis lung cancer tissues and LAK cells from spleen of tumor bearing mouse were irradiated with different low doses of X rays and were cultured after irradiation. Results Low dose ionizing radiation improved the amplification volume of LAK/TIL cells,decreased the cell death ratio in amplification process,and increased the toxicity of LAK/TIL cells. Conclusions Low dose ionizing radiation can result in amplification of biologically activated lymphocytes,and decreases the death ratio of the cells in amplification process.