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| 基因枪介导单纯疱疹病毒DNA疫苗的免疫效果研究 | |
| 引用本文： | 杨慧兰,韩双艳,王捷,杨传红,杨太成. 基因枪介导单纯疱疹病毒DNA疫苗的免疫效果研究[J]. 中国免疫学杂志, 2004, 20(11): 748-750 |
| 作者姓名： | 杨慧兰 韩双艳 王捷 杨传红 杨太成 |
| 作者单位： | 1. 广州军区广州总医院皮肤科,广州,510010 2. 广州军区广州总医院医学实验科,广州,510010 |
| 基金项目： | 国家自然科学基金资助项目 (3 0 2 71192 ) |
| 摘 要： | 目的：研究单纯疱疹病毒DNA疫苗经基因枪免疫小鼠的效果。方法：基因枪免疫小鼠腹部，免疫组化法检测gD抗原在接种部位的表达；ELISA检测血清中抗gD抗体；病毒攻击检测经基因枪免疫的DNA疫苗对动物的保护效果。结果：基因枪可有效将DNA质粒运送至小鼠真皮内，单纯疱疹病毒Ⅱ型gD2糖蛋白基因在真皮和肌肉组织中高效表达，免疫小鼠体内产生高滴度的中和抗体，能有效抵抗HSV-2病毒的攻击。结论：疱疹病毒gD DNA疫苗通过基因枪接种方式，可在小鼠体内产生特异性保护作用。 |
| 关 键 词： | 基因枪接种 中和抗体 病毒攻击 |
| 文章编号： | 1000-484X(2004)11-0748-03 |
Study of immune effect of herpes simplex virus DNA vaccine medicated by gene gun |
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| YANG Hui-Lan,HAN Shuang-Yan,WANG Jie,YANG Chuan-Hong,YANG Ta i-Cheng. Study of immune effect of herpes simplex virus DNA vaccine medicated by gene gun[J]. Chinese Journal of Immunology, 2004, 20(11): 748-750 | |
| Authors: | YANG Hui-Lan HAN Shuang-Yan WANG Jie YANG Chuan-Hong YANG Ta i-Cheng |
| Affiliation: | YANG Hui-Lan,HAN Shuang-Yan,WANG Jie,YANG Chuan-Hong,YANG Ta i-Cheng.Department of Dermatology,Guangzhou General Hospital of Guangzhou Milit ary Command,Guangzhou 510010,China [ |
| Abstract: | Objective:To study effect of DNA immunization mediated by gene gun.Methods:DNA vaccine were used to vaccinate the abdomens of BALB/C mice mediated by gene gun.gD expression at inoculation sites were detected by immuno-histological exa mination and IgG induced were investigated with ELISA.The protective effect of D NA vaccine was assayed too.Results:The DNA vaccine pcDNA3-gD can be successfully delivered to curis and muscle cel ls near inoculation sites mediated by gene gun,and induce high IgG titer in BALB /C mice.The DNA vaccine also can protect the animals from lethal virus challenge .Conclusion:gD DNA vaccine can produce specific protection in mice with gene gun inoculation . [ |
| Keywords: | Gene gun inoculation Neutralization antibody Virus challenge |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |