| UMR106细胞膜钾离子通道的记录及鉴定方法研究 |
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| 引用本文： | 刘彬,张维娟,康玉华,王永毅.UMR106细胞膜钾离子通道的记录及鉴定方法研究[J].河南大学学报(医学版),2002,21(1):1-3. |
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| 作者姓名： | 刘彬 张维娟 康玉华 王永毅 |
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| 作者单位： | 1. 河南大学医学院,生物化学教研室,河南,开封,475001
2. 河南大学淮河医院,内科,河南,开封,475001 |
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| 基金项目： | 河南省自然科学基金资助项目 (994 0 2 2 10 0 ) |
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| 摘 要： | 目的 :建立一种研究大鼠成骨肉瘤细胞 (UMR10 6 )钾离子通道的方法。方法 :采用膜片钳技术 ,在细胞贴附方式下用内面向外的方法记录。并用TTX、CdCl2 分别阻断电压依赖性钠通道和钙通道。结果 :首次在URM10 6细胞上记录到一种电导为 8.79ps电压依赖性钾通道的通道电流。并观察了开放时间、关闭时间以及开放概率随电压的变化。结论 :通过使用钾通道阻断剂证实本方法记录的是一种电压依赖性内向整流型钾通道。
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| 关 键 词： | 离子通道 膜片钳 细胞 |
| 文章编号： | 1008-2867(2002)01-0001-03 |
| 修稿时间： | 2001年11月12 |
Study for Recording and Identifying K+ Channel in Rat Osteoblast-like Cell UMR106 |
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| LIU Bin,ZHANG Wei_juan,KANG Yu_hua,et al.Study for Recording and Identifying K+ Channel in Rat Osteoblast-like Cell UMR106[J].Journal of Henan University,2002,21(1):1-3. |
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| Authors: | LIU Bin ZHANG Wei_juan KANG Yu_hua |
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| Abstract: | Objective: To set up a method to study K + channel in rat osteoblast_like cell(UMR106).Methods: Using cell_attached and inside_out configurations, an inward_rectifying current was recorded. At the meantime, TTX and CdCl 2 were used to block voltage_dependent Na + Channel and Ca 2+ channel.Results: Voltage_dependent potassium current (8.79ps) was successfully recorded on URM106 cells, and Its open times, close time and the change of open probabilities with voltage were observed.Conclusion: It is comfirmed that the channel was a voltage_dependent, inward rectified through using K + channel blockers K + channel. |
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| Keywords: | Ion channel Patch clamp cell |
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