溃疡性结肠炎患者周围血中性粒细胞凋亡和Bcl-2基因的表达

目的　观察溃疡性结肠炎 (UC)患者周围血中性粒细胞 (PMN)凋亡及Bcl 2基因表达的变化。方法　采用流式细胞术检测 18例UC患者周围血PMN凋亡及Bcl 2基因的表达。结果　活动期UC患者PMN凋亡率明显低于健康人对照组和缓解期UC患者 (P <0 .0 1) ,Bcl 2基因的表达明显高于对照组和缓解期患者 (P <0 .0 1)。缓解期UC患者PMN凋亡率与对照组无差别 ,但Bcl 2基因的表达与正常人对照组比较差别有显著性意义 (P <0 .0 1)。不同病情活动期UC患者PMN凋亡及Bcl 2的表达有显著性差异 (P <0 .0 1或P <0 .0 5 )。结论　活动期UC患者周围血PMN凋亡延迟 ,且与病情及疗效密切相关。PMNBcl 2基因表达上调可能是其凋亡延迟的重要分子机制之一。

Apoptosis and expression of Bcl-2 gene of neutrophil in patients with ulcerative colitis

Objective To observe the changes of apoptosis and expression of Bcl-2 gene of neutrophil (PMN) in patients with ulcerative colitis (UC).Methods The apoptosis and expression of Bcl-2 gene of PMN in patients with UC were determined with flow cytometry.Results The apoptosis percentage of PMN in active UC was obviously lower than that of controls and remission UC, but the expression of Bcl-2 of PMN in active UC was higher than that of the other two groups. There was no differential significance of apoptosis of PMN between controls and remission UC, but the expression of Bcl-2 of PMN in remission UC was higher than that of controls.Significant differences of PMN apoptosis and expression of Bcl-2 were found among the different clinical stages of active UC.Conclusion The data demonstrated a delay in apoptotic process of PMN in active UC which correlated with the clinical stage and curative effect. The up regulation of the expression of Bcl-2 gene may be one of the molecular mechanism of the delayed apoptosis of PMN in UC.