ABCG2蛋白在肝癌中表达的临床意义

目的 探讨ABCG2蛋白在肝癌中表达的临床意义.方法 收集2005年1月至2006年12月中山大学附属第一医院165例肝癌患者手术切除的组织标本和25例癌旁正常肝组织及加例肝硬化组织标本,采用免疫组织化学法检测各组织中ABCG2蛋白的表达,并分析与临床病理特征之间的关系.计数资料采用X2检验,Kaplan-Meier法计算生存率,Log-rank法比较组间差异.结果 ABCG2蛋白在正常肝组织及肝硬化组织中均有表达,呈弱阳性.在肝癌组织中,ABCG2表达呈弱阳性者99例、强阳性者66例.ABCG2表达水平与肿瘤直径及数目、周围脏器侵犯及TNM分期有关(χ2=8.130,14.279,4.820,21.179.P<0.05).Kaplan.Meier生存分析显示ABCG2表达强阳性者3年生存率为24.1%,弱阳性者为39.4%,两者比较差异有统计学意义(χ2=15.716,P<0.05).结论 ABCG2在肝癌组织中表达水平与肿瘤侵袭性、TNM分期及患者预后相关,可望成为肝癌新的治疗靶点.

Expression and clinical significance of ABCG2 protein in hepatocellular carcinoma

Objective To investigate the expression and clinical significance of ABCG2 protein in hepato-cellular carcinoma (HCC). Methods Specimens of HCC were collected at The First Aifiliated Hospital of Sun Yat-sen University from January 2005 to December 2006. The expression of ABCG2 protein in 165 samples of HCC tissue, 25 samples of normal liver tissue and 40 samples of cirrhotic liver tissue was detected using immunohisto-chemistry. The correlation between the expression of ABCG2 protein and clinicopathological characters was then analyzed. Enumeration data, survival rate and the difference between groups were analyzed with a chi-square test, the Kaplan-Meier method and Log-rank test, respectively. Results ABCG2 protein expression was weakly posi-tive in all normal and cirrhotic liver tissues. In HCC tissues, the expression of ABCG2 protein was strongly positive in 66 cases and weakly positive in 99 cases. The expression of ABCG2 protein was related to tumor diameter, tumor number, adjacent organ invasion and TNM stages (χ2 =8. 130, 14. 279, 4. 820, 21. 179, P <0. 05). Kaplan-Meier survival analysis revealed that patients with strongly positive ABCG2 protein had a significantly lower 3-year overall survival (24. 1%) compared with those with weakly positive ABCG2 protein (39. 4%) (χ2 = 15.716, P<0.05). Conclusions The expression level of ABCG2 protein is related to tumor invasiveness, TNM stage and prognosis. ABCG2 has the potential to become a new target for HCC treatment.