变应性鼻炎患者外周血白细胞及鼻黏膜诱导型一氧化氮合酶mRNA 表达的意义

目的　探讨变应性鼻炎 (allergicrhinitis,AR)患者外周血白细胞及鼻黏膜诱导型一氧化氮合酶 (induciblenitricoxidesynthase,iNOS)mRNA表达的关系。方法　选择 3 5例AR患者及 3 0例健康人的外周血白细胞。其中 8例变应性鼻炎患者鼻黏膜 ,6例正常鼻黏膜。iNOS mRNA表达采用原位杂交方法。血浆一氧化氮 (nitricoxide,NO)水平采用硝酸还原酶比色法测定。结果　健康人外周血白细胞未见iNOS mRNA表达 ,而AR患者外周血白细胞iNOS mRNA表达高度增强 ,其阳性率达 40 82 %。正常人鼻黏膜上皮、腺体及巨噬细胞可见iNOS mRNA的低度表达 ,而AR患者中上皮、腺体及巨噬细胞增生 ,并iNOS mRNA表达高度增强 (t=2 3 17,P <0 0 0 1)。AR组血浆NO水平显著高于对照组 (t=2 7 89,P <0 0 1)。结论　AR患者血浆NO水平与外周血白细胞及组织内iNOS mRNA表达高度增强有关。提示iNOS NO通路在AR的发病过程中可能起重要作用。本研究为检测体内某些信号提供了简便易行的原位杂交试验方法

Role of expression inducible nitric oxide synthase-mRNA of leukocyte and nasal mucosa in allergic rhinitis

Objective To investigate the relationship between expression of leukocyte inducible nitric oxide synthase (iNOS) mRNA and allergic rhinitis (AR) Method Thirty five patients with AR and 30 healthy controls were included in this study Expression of iNOS mRNA in peripheral blood leukocyte was detected by in situ hybridization NO in plasm was measured by nitrate reductase Expression of iNOS mRNA in nasal mucosal was detected in 8 patients with AR and 6 healthy controls Result No expression of lekocyte iNOS mRNA in healthy controls was found In AR patients, the positive cells were significantly increased, the positive rate reached 40 82% Expression of iNOS mRNA was localized at the epithelium,gland and macrophage in healthy controls Hyperplasia and expression of iNOS mRNA increased at epithelium,gland and macrophage in the AR patients( t =23 17, P <0 001) The level of plasm NO in AR group was higher than that in healthy control group ( t =27 89, P <0 001) Conclusion There is a relationship between expression of leukocyte iNOS mRNA and the level of plasm NO in AR patients The study provides an easy method of in situ hybridization for detecting some signal in body