| 分泌抗破伤风类毒素人单克隆抗体的人鼠杂交瘤细胞系的建立 |
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| 引用本文： | 叶银英,刘立华,姜凯,葛曼丽,李明举.分泌抗破伤风类毒素人单克隆抗体的人鼠杂交瘤细胞系的建立[J].东南大学学报(医学版),1991(4). |
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| 作者姓名： | 叶银英 刘立华 姜凯 葛曼丽 李明举 |
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| 作者单位： | 南京铁道医学院医学科学研究所，南京铁道医学院医学科学研究所，南京铁道医学院医学科学研究所，南京铁道医学院医学科学研究所，南京铁道医学院医学科学研究所 |
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| 摘 要： | 作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合,对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。按免疫时间(天)及体外刺激与否分为四组、其中以 PWM+TT 体外刺激 PBL(比体外不刺激)的细胞融合率高,且易建成稳定分泌抗体的细胞系。经5次克隆化后获得5株抗 TT 的人单抗杂交瘤细胞系。经双扩确定 IgG2株,IgM3株。冻存10个月后复苏,传代及适当的克隆化后仍为阳性。上清液中人 IgM 的分泌量为0.42～1.15μg/ml。经染色体鉴定确为人-鼠杂种细胞。
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| 关 键 词： | 人-鼠杂交瘤 人单克隆抗体 破伤风类毒素 |
ESTABLISHMENT OF HUMAN MOUSE HETEROHYBRIDOMA CELL LINES SECRETING HUMAN MONOCLONAL ANTIBODIES AGAINST TETANUS TOXOID |
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| Ye Yinying Liu Lihua Ge Manli Jiang Kai Li Mingju.ESTABLISHMENT OF HUMAN MOUSE HETEROHYBRIDOMA CELL LINES SECRETING HUMAN MONOCLONAL ANTIBODIES AGAINST TETANUS TOXOID[J].Journal of Southeast Univ: Medical Sci Ed,1991(4). |
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| Authors: | Ye Yinying Liu Lihua Ge Manli Jiang Kai Li Mingju |
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| Abstract: | The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).Four experimental groups were divided by immunization time and presence or absence of stimulation in vitro.The group with pokeweed mitogen plus TT stimulation in vitro has the highest fusion rate and easily establishes cell line secreting antibodies stably.Five hybridoma cell lines secreting human monoclonal antibodies against tetanus toxoid were obtained after clonization for five times Double diffusion assay proves that two of the ceil lines secret IgG and three secret IgM.They produced antibodies against TT after freezing for 10 months,revivification,generation and clonization. Karyotype analysis confirmes that they are human-mouse hybridomas. |
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| Keywords: | heterohybridomas human monoclonal antibodies tetanus toxoid |
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