补肾化痰中药对慢性支气管炎小鼠垂体-性腺-免疫轴的影响

目的：研究补肾化痰中药对慢性支气管炎（慢支）小鼠垂体－性腺－免疫轴的影响。方法：建立烟熏致小鼠慢支模型，并设正常对照组、补肾化痰中药大、小剂量组和桂龙咳喘宁组，测定各组小鼠血清睾酮（T）、促黄体生成素（LH）、白细胞介素－2（IL－2）、白细胞介素－8（IL－8）水平和睾丸、附睾、胸腺的脏器指数，观察睾丸组织的显微结构。结果：小鼠慢支模型组与正常对照组比较血清T、IL－2水平和睾丸、附睾、胸腺的脏器指数均有明显下降，血清IL－8水平升高，睾丸出现萎缩性改变。补肾化痰中药组各项指标均有不同程度改善。结论：烟熏致慢支小鼠伴有生殖内分泌功能紊乱和免疫功能低下，肾补化痰中药通过纠正紊乱的性激素水平和睾丸的病理性改变以及提高机体的免疫力，对垂体－性腺－免疫轴起到调节作用。

Effect of Chinese Bushen Huatan Drugs on Pituitary-Sex Gland-Immune Axis in Smoking Induced Chronic Bronchitis Mice

Objective: To study the effect of Chinese Bushen Huatan Drugs (BSHT, Chinese drugs for Kidney tonifying and phlegm resloving) on pituitary sex gland immune axis in smoking induced mice model of chronic bronchitis. Methods: Model mice were induced by smoking and divided into the model control group, the large dosage of BSHT group, the small dosage of BSHT group and the positive control group (treated by Guilong Kechuanning, a proved effective Chinese patent drug). A blank group of normal mice was also set for control. Serum levels of testosterone (T), luteotropic hormone (LH), interleukin 2 (IL 2), interleukin 8 (IL 8), visceral indexes of testis, epididymis and thymus were measured and microstructure of testis tissue was also observed. Results: As compared with those in the blank group, serum T, LH and IL 2 levels, and testis, epididymis and thymus indexes were all lower, and IL 8 level was higher in the model control group, moreover, atrophic change of testis was present in the model mice. These abnormal changes were all improved in the BSHT groups. Conclusion: Smoking induced mice model of chronic bronchitis, which may cause reproductive endocrine disturbance and immunosuppression. BSHT could modulate the pituitary sex gland immune axis through adjusting the disturbed sex hormone, improve the pathological change of testis and enhance the immunity of organism.