An electron microscopic and immunohistochemical study of Merkel cell carcinoma |
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Authors: | Masayori Kagoura Shigeaki Sakita Takashi Toyomoto and Masaaki Morohashi |
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Institution: | (1) Department of Dermatology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, 930-01 Toyama, Japan |
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Abstract: | Two cases of Merkel cell carcinoma (MCC) were examined by electron microscopy and immunohistochemistry. Histologically, tumor cells, which extended from the dermis into the subcutis, showed anastomosing bands with partial trabecular pattern. The ultrastructural study showed tumor cells in case 1 with numerous neurosecretory granules. The number of granules in case 2, however, was smaller compared with that in case 1. Perinuclear bundles of filaments were present in case 2, but few bundles were observed in case 1. By immunohistochemistry, cytokeratin (CK)-8, -18, -19, and -20 and epithelial membrane antigen were stained positively within tumor cells in both cases. It was interesting that staining patterns of chromogranin A and of neuron-specific enolase were different in the two cases. These data indicated that CK-20 is a useful marker for diagnosing MCC and that ultrastructural and immunohistochemical differences in both cases were the result of phenotypic variation. |
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Keywords: | Merkel cell carcinoma Dense core granule Chromogranin A Electron microscopy Immunohistochemistry study |
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