| 羊耳菊提取物在大鼠粪便中的代谢产物分析 |
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| 引用本文： | 巩仔鹏,吴林霖,伍萍,李梅,侯靖宇,陈亭亭,兰燕宇,李勇军,李月婷,王永林.羊耳菊提取物在大鼠粪便中的代谢产物分析[J].中国实验方剂学杂志,2017,23(24):100-105. |
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| 作者姓名： | 巩仔鹏 吴林霖 伍萍 李梅 侯靖宇 陈亭亭 兰燕宇 李勇军 李月婷 王永林 |
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| 作者单位： | 贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 贵州省药物制剂重点实验室, 民族药与中药开发应用教育部工程研究中心, 药学院, 国家苗药工程技术研究中心, 贵阳 550004 |
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| 基金项目： | 国家自然科学基金项目（81360680）；贵州省优秀青年科技人才培养项目（黔科合人字（2015）11号）；贵州省教育厅民族药与中药开发应用产学研基地建设项目（黔科合KY字[2013]122） |
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| 摘 要： | 目的:研究羊耳菊提取物给药后在大鼠粪便中的代谢产物,为该药材的后续研究与开发提供参考。方法:采用超高效液相色谱-三重四级杆飞行时间质谱(UPLC-Q-TOF-MS/MS)检测大鼠口服羊耳菊提取物后粪便中的代谢产物,每次给药剂量100 g·kg~(-1)。使用RRHD Eclipse Plus C_(18)色谱柱(2.1 mm×100 mm,1.8μm),流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液梯度洗脱,选择电喷雾离子源,负离子模式进行扫描,准确质量测定采用甲酸钠校正标准液,采用Metabolite Detect(micr OTOF 2.3)等软件进行数据处理,鉴定代谢产物的化学结构。结果:在大鼠粪便中检测到原型M8(1,3-O-二咖啡酰基奎宁酸),M1~M4(二氢单咖啡酰基奎宁酸异构化产物),M5(二氢咖啡酸的硫酸酯化产物),M6~M7(单咖啡酰基奎宁酸的甲基化产物)等22个代谢产物。结论:羊耳菊提取物给药后,咖啡酰基奎宁酸类活性成分在大鼠粪便中的代谢途径以甲基化、还原反应为主。
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| 关 键 词： | 羊耳菊 提取物 咖啡酰基奎宁酸 代谢产物 超高效液相色谱-三重四级杆飞行时间质谱 绿原酸 |
| 收稿时间： | 2017/5/27 0:00:00 |
Analysis of Metabolites of Inula cappa Extract in Rat Feces |
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| GONG Zi-peng,WU Lin-lin,WU Ping,LI Mei,HOU Jing-yu,CHEN Ting-ting,LAN Yan-yu,LI Yong-jun,LI Yue-ting and WANG Yong-lin.Analysis of Metabolites of Inula cappa Extract in Rat Feces[J].China Journal of Experimental Traditional Medical Formulae,2017,23(24):100-105. |
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| Authors: | GONG Zi-peng WU Lin-lin WU Ping LI Mei HOU Jing-yu CHEN Ting-ting LAN Yan-yu LI Yong-jun LI Yue-ting and WANG Yong-lin |
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| Institution: | National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China and National Engineering Research Center of Miao''s Medicines, School of Pharmacy, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Guizhou Medical University, Guiyang 550004, China |
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| Abstract: | |
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| Keywords: | Inula cappa extract caffeoylquinic acids metabolites UPLC-Q-TOF-MS/MS chlorogenic acid |
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