扶肾颗粒改善腹膜超滤功能的分子机制

目的:观察扶肾颗粒对腹膜透析大鼠的腹膜超滤功能、乙二醛酶-1(glyoxalase 1,Glo-1)及终末期糖基化产物(advanced glycation end products,AGEs)表达的影响。方法:建立5/6肾切除肾衰模型,将造模成功的的SD大鼠随机分为1.5%腹透液(peritonealdialysis solution,PDS)模型组;1.5%PDS+扶肾颗粒干预组;4.25%PDS模型组;4.25%PDS+扶肾颗粒干预组,每组17只;另设空白组,15只,4周后处死,取各组大鼠腹膜组织及血清,计算腹膜超滤量,蛋白免疫印迹法(Western blot)检测大鼠腹膜组织Glo-1蛋白表达水平,酶联免疫吸附法(ELISA)检测血清AGEs的含量。结果:扶肾颗粒干预组腹膜超滤量、血清AGEs含量比同浓度透析模型组均降低(P0.05,P0.01)。与空白组比较,模型组Glo-1蛋白表达增加(P0.05);1.5%PDS模型组Glo-1蛋白表达水平虽总体高于同浓度PDS药物干预组,但无明显统计学差异;4.25%PDS模型组与同浓度PDS药物干预组比较,Glo-1蛋白表达水平显著增高(P0.05)。结论:扶肾颗粒可以调节Glo-1的表达水平并减少血清AGEs的蓄积,从而保护残肾功能,延缓腹膜超滤衰竭及腹膜纤维化。

Molecule Mechanism of Fushen Granules in Improving Peritoneal Ultrafiltration Function

Objective: To observe the effect of Fushen granules on the peritoneal ultrafiltration function, glyoxalase-1 (Glo-1) and advanced glycation end products (AGEs) in rats with peritoneal dialysis. Method: The chronic renal failure peritoneal dialysis rat model was induced through 5/6 nephrectomy. After successful modeling, the SD rats were randomly divided into 1.5% PD model group; 1.5% PD+Fushen granules intervention group; 4.25% PD model group; and 4.25% PD+Fushen granules intervention group (group E), with 17 rats in each group; the control group was also set up, with 15 rats. After 4 weeks, the rats were put to death, and their peritoneal tissues and serum were collected to calculate the peritoneal ultrafiltration volume; Western blot was used to detect the rat peritoneal organization Glo-1 protein expression. And enzyme-linked immunoassay (ELISA) was used to detect the content of serum AGEs. Result: Peritoneal ultrafiltration and serum AGEs content in Fushen granules intervention group were lower than dialysis model group of the same concentration, with statistically significant differences (P<0.05, P<0.01). Compared with the control group, Glo-1 protein content increased in the model group, with statistically significant differences (P<0.05). Glo-1 protein expression in group B was higher than that in group C, but with on statistically significant difference. Compared with group E, Glo-1 protein expression was significantly elevated, with statistically significant differences (P<0.05). Conclusion: Fushen granules can regulate the expression of Glo-1 and reduce the accumulation of serum AGEs, so as to protect the residual kidney function and delay peritoneal filtration and peritoneal fibrosis.