四效散对糖尿病足溃疡大鼠创面愈合的影响

目的:观察四效散在糖尿病足溃疡大鼠创面组织愈合过程中所起到的作用,探究四效散对糖尿病溃疡大鼠创面愈合过程中的影响及作用机制。方法:将SPF级SD大鼠随机分成4组,分别为空白组(普通大鼠),模型组,甲硝唑(5 m L·kg~(-1)·d-1)组,四效散(100 mg·kg~(-1)·d-1)组。甲硝唑组予甲硝唑注射液外敷,四效散组予四效散外敷,治疗14 d后,检测大鼠血清中表皮生长因子(epidermal growth factor,EGF)及碱性成纤维细胞生长因(basic fibroblast growth factor,b FGF)含量;免疫组化检测创面肉芽组织中成纤维细胞数及新生毛细血管数;苏木素-伊红(HE)染色观察组织形态学变化。结果:与模型组比较,甲硝唑组、四效散组大鼠血清中b FGF,EGF含量显著升高(P0.01),其中四效散组大鼠血清b FGF,EGF的升高优于甲硝唑组(P0.01)。与模型组比较,甲硝唑组、四效散组的新生毛细血管数密度及成纤维细胞数显著升高(P0.05),四效散组新生毛细血管数密度及成纤维细胞数的增多高于甲硝唑组(P0.05)。结论:四效散能够提高大鼠血清中b FGF,EGF蛋白水平,有利于成纤维细胞、新生毛细血管的增殖,有利于创面肉芽组织的生长,从而促进糖尿病足溃疡的愈合。

Effect of Sixiaosan on Wound Healing of Diabetes Ulcer Rats

Objective: To observe the effect and mechanism of Sixiaosan on wound tissue healing of diabetic foot ulcer in rats. Method: SPF SD rats were randomly divided into 4 groups: blank group (normal rats), model control group, metronidazole group (5 mL · kg^-1 · d^-1) and Sixiaosan group (100 mg · kg^-1 · d^-1). Metronidazole injection was applied in metronidazole group for external use, Sixiaosan was provided to Sixiaosan group for external use. At 14 d after treatment, serum epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were detected. Immunohistochemistry was used to detect the numbers of fiber cells and newborn blood capillaries in wound granulation tissues; hematoxylin-eosin (HE) staining was used to observe histomorphological changes. Result: In metronidazole and Sixiaosan groups, bFGF and EGF in serum of rats were significantly increased (P<0.01). Specifically, bFGF and EGF in Sixiaosan group increased higher than those of metronidazole groups (P<0.01). In metronidazole and Sixiaosan groups, the number and density of newborn blood capillaries and the number of fibroblasts were significantly increased (P<0.05). In Sixiaosan group, the number and density of newborn blood capillaries and the number of fibroblasts increased higher than those of metronidazole groups (P<0.05). Conclusion: Sixiaosan is able to improve the levels of bFGF and EGF in serum of rats, and conducive to the proliferation of fibroblasts and newborn blood capillaries, and the growth of wound granulation tissues, so as to promote the healing of diabetic foot ulcer.