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| 舌鳞癌细胞对Iressa的敏感性及其表皮生长因子受体突变 | |
| 引用本文： | 孙传政,陈福进,曾木圣,宋立兵,李满枝,曾宗渊.舌鳞癌细胞对Iressa的敏感性及其表皮生长因子受体突变[J].华中科技大学学报(医学版),2011,40(1):41-46. |
| 作者姓名： | 孙传政 陈福进 曾木圣 宋立兵 李满枝 曾宗渊 |
| 作者单位： | 1. 昆明医学院第三附属医院头颈外科,昆明,650118;中山大学肿瘤防治中心头颈外科,广州,510060;华南肿瘤学国家重点实验室,广州,510060 2. 中山大学肿瘤防治中心头颈外科,广州,510060;华南肿瘤学国家重点实验室,广州,510060 3. 华南肿瘤学国家重点实验室,广州,510060 |
| 基金项目： | 云南省科技厅基础应用研究面上项目(No.2007C149M); 云南省教育厅科学研究基金项目(No.07Y40515)资助 |
| 摘 要： | 目的研究中国人舌鳞癌细胞系对表皮生长因子受体(EGFR)抑制剂Iressa的敏感性及其EGFR突变,初步探讨Iressa在头颈鳞癌治疗中的价值。方法以中国人舌鳞癌细胞系Tscca和Tca8113为研究对象,采用MTT法检测Iressa对其增殖的抑制作用;流式细胞仪检测细胞周期和凋亡;对Tscca和Tca8113进行EGFR外显子18～21测序。结果 Iressa对Tscca、Tca8113均有显著的增殖抑制作用,呈剂量依赖性。细胞周期分析显示,较低浓度Iressa(18.2μmol/L和36.4μmol/L)可使Tscca和Tca8113细胞G0/G1期比例增高;流式细胞仪分析显示高浓度Iressa可诱导Tscca和Tca8113细胞凋亡。测序发现Tscca和Tca8113的EGFR外显子20存在同一静止突变,即2361G-A,Q787Q。结论 Iressa可抑制Tscca和Tca8113的增殖,呈剂量依赖性;较低浓度时诱导细胞周期G0/G1期比例增高,高浓度时诱导凋亡,其抑制作用与EGFR突变未见相关性。 |
| 关 键 词： | 舌鳞癌 表皮生长因子受体 Iressa |
Sensitivity of Tongue Squamous Cell Carcinoma to Iressa and Its EGFR Mutation |
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| Sun Chuanzheng,Chen Fujin,Zeng Musheng et al.Sensitivity of Tongue Squamous Cell Carcinoma to Iressa and Its EGFR Mutation[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(1):41-46. | |
| Authors: | Sun Chuanzheng Chen Fujin Zeng Musheng |
| Institution: | Sun Chuanzheng1,2,3,Chen Fujin2,Zeng Musheng3 et al1Department of Head and Neck Surgery,Third Affiliated Hospital of Kunming Medical College,Kunming650118,China2Departmemt of Head and Neck Surgery,Cancer Center,SUN Yat-sen University,Guangzhou 510060,China3State Key Laboratory of Oncology in Southern China,China |
| Abstract: | Objective To evaluate the effect of Iressa on squamous cell carcinoma lines Tca8113,Tscca of tongue and study the mutation statuses of EGFR gene in them.Methods The effect of Iressa on Tscca and Tca8113 cells was detected by MTT assay.The distribution of cell cycle and apoptosis rate were assayed by flow cytometry and Hoechst staining respectively.The sequence of EGFR exons 18-21 of the two cell lines was analyzed.Results The proliferation of Tscca and Tca8113 cells was inhibited by Iressa in a dose-depende... |
| Keywords: | tongue squamous cell carcinoma epidermal growth factor receptor Iressa |
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