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| siRNA沉默Survivin抑制结肠癌细胞株HCT116增殖并增强其对奥沙利铂的敏感性 | |
| 引用本文： | 王强,舒榕,刘冬玲,刘静,王蕾蕾.siRNA沉默Survivin抑制结肠癌细胞株HCT116增殖并增强其对奥沙利铂的敏感性[J].华中科技大学学报(医学版),2011,40(1). |
| 作者姓名： | 王强 舒榕 刘冬玲 刘静 王蕾蕾 |
| 作者单位： | 1. 武汉科技大学医学院基础部免疫学教研室,武汉,430086 2. 湖北省中山医院麻醉科,武汉,430030 3. 武汉市普爱医院病理科,武汉,430030 |
| 基金项目： | 湖北省科技计划自然科学基金面上项目(2010CDB03503); 武汉科技大学青年科技骨干培育计划资助项目(2009xz8) |
| 摘 要： | 目的研究Survivin特异性siRNA对人结肠癌细胞株HCT116增殖和对化疗药物奥沙利铂敏感性的影响。方法体外将Survivin特异性siRNA表达载体pgsiRNA-Survivin转染HCT116细胞,通过Western blot和Real time-PCR方法检测细胞Survivin的表达,采用流式细胞术分析细胞周期分布,MTT法检测干扰Survivin后HCT116细胞对奥沙利铂的敏感性。结果 pgsiRNA-Survivin转染HCT116细胞48 h后,细胞的Survivin基因表达明显降低,导致了HCT116细胞G2/M期阻滞;pgsiRNA-Survivin转染组细胞对奥沙利铂的敏感性显著性增强。结论 Survivin特异性siRNA能显著沉默HCT116细胞Survivin基因,抑制细胞增殖,并增强HCT116细胞对奥沙利铂的敏感性。 |
| 关 键 词： | Survivin RNA干扰 结肠癌 HCT116细胞 细胞增殖 奥沙利铂 |
Survivin Special siRNA Inhibited Proliferation of Human Colon Cancer HCT116 Cells and Enhanced Their Sensitivity to Oxaliplatin |
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| Wang Qiang,Shu Rong,Liu Dongling et al.Survivin Special siRNA Inhibited Proliferation of Human Colon Cancer HCT116 Cells and Enhanced Their Sensitivity to Oxaliplatin[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(1). | |
| Authors: | Wang Qiang Shu Rong Liu Dongling |
| Institution: | Wang Qiang1,Shu Rong2,Liu Dongling3 et al1Department of Immunology,Medical College of Wuhan University of Science and Technology,Wuhan 430086,China2Department of Anesthesiology,Hubei Zhongshan Hospital,Wuhan 430030,China3Department of Pathology,Wuhan Puai Hospital,China |
| Abstract: | Objective To evaluate the effect of Survivin special siRNA on proliferation of colon cancer HCT116 cells and their sensitivity to oxaliplatin.Methods The expression vector of pgsiRNA-Survivin was constructed and transfected into HCT116 cells by Lipofectin.The protein and mRNA expression of Survivin was detected by Western blot and real-time PCR.The changes of cell cycle and sensitivity to oxaliplatin after transfection were examined by flow cytometry and MTT assay,respectively.Results In HCT116 cells,the pr... |
| Keywords: | Survivin RNA interference colon cancer HCT116 cell cell proliferation oxaliplatin |
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