加味茵陈汤含药血清制备及其对体外肝细胞损伤模型的作用

目的：探索加味茵陈汤含药血清的制备方法及其对体外培养大鼠肝细胞损伤模型的作用。方法：制备加味茵陈汤（茵陈、栀子、大黄、三七、丹参、赤芍、生地、丹皮、白芍、柴胡）汤剂（2 g生药/ml）;大鼠中药灌胃（30 g/kg,2次/d,连续3 d）制备药物血清;改进Seglen胶原酶原位灌注分离大鼠肝细胞,培养体系中加入D-氨基半乳糖（25μg/ml）诱导肝细胞损伤模型,以不同浓度（5%、10%1、5%）加入药物血清培养24 h,于培养1、36、1、2和24 h取上清液测定ALT、AST和LDH,同步测定肝细胞的MTT反应,并于倒置相差显微镜下观察肝细胞形态变化。结果：从各时间点观察,随含中药血清浓度的增加,肝细胞培养上清液ALT、AST、LDH水平逐渐下降,10%药物血清与15%药物血清剂量组之间在各时间点比较无统计学差异（P〉0.05）,其余各组比较统计学差异均有显著性（P〈0.05）,培养肝细胞增殖活性则呈升高趋势。结论：大鼠的含中药血清可以降低肝细胞损伤模型AST、ALT及LDH等指标,增加细胞活性指标,随浓度增加而作用增强。

Preparation of modified oriental wormwood decoction containing serum and it's protective effect on necrotic hepatocyte injury models

Objective: To prepare the modified oriental wormwood decoction(MOWD) containing serum,and to study it's protective in vitro effect on necrotic hepatocyte injury models.Methods:MOWD(2 g/ml,including oriental wormwood,capejasmine fruit,rhubarb,notoginseng,dried rehmannia root,moutan bark,red peony root,white peony root,red sage root and astragalusand bupleurum) containing serum was prepared by gavage administration of the decotion to the rats at a dose of 30 g/kg twice a day for three days.Hepatocytes were ob...