高效液相色谱法测定胰必清颗粒中有效成分含量

［摘要］目的建立一种高效液相色谱（HPLC）法，测定胰必清颗粒中大黄素、大黄酚、厚朴酚等成分。方法采用Hypersil ODS色谱柱,流动相为甲醇 0.5%高氯酸水溶液(85:15),检测波长254 nm。结果大黄素、芦荟大黄素、大黄酚、大黄酸、厚朴酚及和厚朴酚的加样回收率(n=5)分别为: 99.62%, 96.60%, 98.69%, 103.70%, 99.84%, 101.30%； 精密度RSD(n=5)分别为1.18%，2.34%，1.85%，2.45%，0.61%，1.21%。结论该方法简便，快速，结果准确可靠，灵敏度高、重现性好,可作为胰必清颗粒质量控制的定量方法。

Content Determination of the Active Principles in Yibiqing Granules with HPLC

Objective To set up an HPLC method for the content determination of emodin,aloe-emodin,chrysophanol,rhein,magnolol and honokiol in the yibiqing granules. Methods The determination was performed on a Hypersil ODS column with methanol-0.5%HClO4(85:15) as the mobile phase and the detection wavelength was 254 nm. Results The average rates of recovery(n=5) of emodin,aloe-emodin,chrysophanol,rhein,magnolol and honokiol were 99.62%,96.60%,98.69%,103.70%,99.84%,and 101.30%,respectively;the RSD(n=5) being 1.18%,2.34%,1.85%,2.45%,0.61% and 1.21%,respectively. Conclusion The method was shown to be handy,rapid,sensitive,accurate and well reproducibile.It can be used as a quantitative method for the quality control of yibiqing granules.