| MC3T3-HA复合培养后的细胞凋亡检测 |
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| 引用本文： | 宋辰刚,邱小忠,吴刚,余磊,赖桂华,欧阳钧. MC3T3-HA复合培养后的细胞凋亡检测[J]. 中国临床解剖学杂志, 2007, 25(5): 567-570 |
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| 作者姓名： | 宋辰刚 邱小忠 吴刚 余磊 赖桂华 欧阳钧 |
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| 作者单位： | 1. 南方医科大学解剖学教研室,广东省组织构建与检测重点实验室,广州,510515
2. 华南理工大学特种功能材料教育部重点实验室,广州,510641 |
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| 基金项目： | 华南理工大学特种功能材料教育部重点实验室开放课题;国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的:探讨羟基磷灰石(Hydroxyapatite,HA)压片材料对小鼠MC3T3成骨细胞凋亡的影响,提供一种方便可行的组压片材料的生物相容性检测方法。方法:小鼠MC3T3成骨细胞株接种在HA压片材料上,应用光学显微镜和PI-Hoechst33342双染色荧光显微镜观察细胞,采用RT-PCR方法分析HA对Id2(Inhibitor of differentiation 2,分化抑制因子2)和OPN(osteopontin,骨桥蛋白)等基因表达的影响。结果:HA压片材料组MC3T3细胞凋亡率(78.7%)显著高于正常对照组(24.6%)(t’=28.1,P=0.000)。HA压片材料双荧光染色可以直接检测种子细胞的凋亡。结论:HA压片可促进小鼠MC3T3成骨细胞凋亡。
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| 关 键 词： | 羟基磷灰石 小鼠MC3T3成骨细胞 凋亡 |
| 文章编号： | 1001-165X（2007）05-0567-04 |
| 修稿时间： | 2007-04-03 |
The detection of apoptosis of mouse MC3T3 cells cultured with hydroxyapatite disks |
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| SONG Chen-gang, QIU Xiao-zhong, WU Gang,et al.. The detection of apoptosis of mouse MC3T3 cells cultured with hydroxyapatite disks[J]. Chinese Journal of Clinical Anatomy, 2007, 25(5): 567-570 |
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| Authors: | SONG Chen-gang QIU Xiao-zhong WU Gang et al. |
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| Affiliation: | Department of Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, China |
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| Abstract: | Objective: To investigate the effect of hydroxyapatite(HA) material on apoptosis of mouse MC3T3 cells, and find a convenient and feasible method for the detection of biocompatibility about HA material. Methods: Mouse MC3T3 cells were cultured on the HA disks, dyed with PI-Hoechst33342 and observed under light microscope and fluorescence microscope. Expression of DNA of Id2 (Inhibitor of differentiation 2) and OPN (osteopontin) was tested through RT-PCR. Results: The rate (78.7%) of apoptosis of mouse MC3T3 cells cultured on HA disks was significantly higher than the rate (24.6%) of the control groups. (t'=28.1, P=0.000). The results implied that double fluorescent dyeing can detect apoptosis of seed cells directly. Conclusions: HA material can promote apoptosis of MC3T3 cells. |
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| Keywords: | hydroxyapatite mouse MC3T3 osteoblast apoptosis |
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