桂附胶囊治疗子宫内膜异位症实验研究

目的探讨桂附胶囊对实验性子宫内膜异位症大鼠激素及相关因素的影响.方法以子宫内膜自体移植法建立大鼠子宫内膜异位症模型.随机分为桂附胶囊组及丹那唑组.放射免疫法测定血清雌二醇(E2)、孕酮(P);免疫组化法测定雌激素受体(ER)、孕激素受体(PR)、血管内皮生长因子(VEGF)、NK细胞.结果桂附胶囊能够降低子宫内膜异位症大鼠外周血中E2、P水平(P＜0.01);降低异位内膜ER、PR的含量(P＜0.05);提高NK细胞活性(P＜0.05);并可抑制血管内皮生长因子(VEGF)的生成.桂附胶囊的实验效果与对照药丹那唑相近.结论桂附胶囊能够通过调节内异症大鼠的内分泌而抑制异位内膜的血管形成,从而抑制异位内膜的生长;同时增加NK细胞的活性,以加强对异位内膜组织的清除作用.

Experimental research of GuiFu capsule in treating endometriosis

Objective: To study the effect of GuiFu capsule on sexual hormone and other relative factors in rats with endometriosis.Methods: Endometriosis was induced in rats by surgery of self-endometrium transplant.Rats with successful models were randomized into GuiFu capsule group and Danazol group.Expressions of ER,PR,VEGF and NK cells were examined by immunohistochemical stainin;the level of E2 and P was detected by radioimmunoassay.Results: GuiFu capsule can reduce the level of E2 and P in the blood of rats(P<0.01);lower the content of ER and PR in ectopia endometrium(P<0.05);raise the activity of NK cells(P<0.05);and restrain the generation of VEGF.The experiment results of Guifu capsule are close to those of Danazol.Conclusion: GuiFu capsule can inhibit the growth of ectopica endometrium by regularing patient's endocrine.At the same time,it increases the activity of NK cells to reinforce their effect of getting rid of the ectopia endometrium.