脾虚型哮喘儿童Th细胞和T细胞亚群的状态研究

目的探讨脾虚型（缓解期）哮喘儿童外周血Th细胞和T细胞亚群的状态及意义。方法设正常对照组（n=38），脾虚型（缓解期）哮喘组（n=17），采用荧光单细胞内染色法检测外周血Th1、Th2细胞活性，采用单克隆抗体间接免疫荧光法检测T细胞亚群，统计检测结果并进行统计分析。结果脾虚型缓解期哮喘组与对照组比较，Th1与对照组比较无显著性差异（P〉0．05），Th2明显升高，Th1／Th2明显降低（P〈0．01）；CD3^＋无显著性差异（P〉0．05），CD4^＋、CD4^＋／CD；均明显降低（P均〈0．01），CD8^＋明显升高（P〈0．01）；结论脾虚型缓解期哮喘儿童存在Th1／Th2细胞偏移和T细胞亚群的紊乱，偏移的原因主要为Th2细胞活性明显升高所致。

Research of Th Cell and T-Cell Subgroups in Asthma of Spleen Deficiency Type in Children

Objective To investigate the state and significance of Th cell and T cell subgroups in peripheral blood in children with asthma of spleen deficiency type（ recovery stage）. Methods Normal control group （ n = 38 ） and asthma group of spleen deficiency type （ recovery stage） （ n = 17 ） were designed. Fluo-rescence mononuclearcytes coloring was used to detect the activity of Thl and Th2 cells in peripheral blood. Monoclonal antibody indirect immunofluorescence was applied to detect T cell subgroup. The statistical analysis was conducted for the detected results. Results Compared with control group, in asthma group of spleen deficiency type（ recovery stage ） , there was no significant difference in Thl （P 〉 0.05 ） , but Th2 increased apparently and Th1/Th2 decreased apparently （ P 〈 0.01 ） ; there was no significant difference in CD3^＋ （ P 〉 0.05 ）, but CD4^＋ and CD^4＋4/CD s decreased apparently （ P 〈 0.01 ）, and CD s increased apparently （ P 〈 0.01 ）. Conclusion The children with asthma of spleen deficiency type （ recovery stage） have the excursion of Th1/Th2 and the disturbance of T cell subgroup. The excursion is mainly induced by the apparent increase of Th2 cell activity.